Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (21): 3342-3348.doi: 10.3969/j.issn.2095-4344.1768

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Effect of concentrated growth factors on proliferation and differentiation of human gingival fibroblasts

Li Xiaoju1, Song Guangbao2, Yang Jianzhen3, Wei Bo1, Wu Bin1   

  1. 1Department of Stomatology, the People’s Hospital of Longhua, Shenzhen 518109, Guangdong Province, China; 2Center of Special Clinic, Guangdong Provincial Stomatological Hospital, Guangzhou 510280, Guangdong Province, China; 3Department of Endodontics, Panyu Branch of Guangdong Provincial Stomatological Hospital, Guangzhou 510280, Guangdong Province, China
  • Revised:2019-04-05 Online:2019-07-28 Published:2019-07-28
  • Contact: Song Guangbao, MD, Chief physician, Professor, Center of Special Clinic, Guangdong Provincial Stomatological Hospital, Guangzhou 510280, Guangdong Province, China
  • About author:Li Xiaoju, Master, Attending physician, Department of Stomatology, the People’s Hospital of Longhua, Shenzhen 518109, Guangdong Province, China
  • Supported by:

    the Medical Science Research Foundation of Guangdong Province, No. A2017540 (to YJZ); the Shenzhen Municipal Health and Family Planning System Research Project, No. SZFZ2018035 (to LXJ)

Abstract:

BACKGROUND: In the blood, there are a large number of growth factors and platelet components with regeneration and repair functions, which can promote the regeneration of soft and hard tissues.
OBJECTIVE: To study the effect of concentrated growth factor extract on the proliferation and osteogenic differentiation of human gingival fibroblasts and to explore the clinical application of concentrated growth factors in oral implantology.
METHODS: Patients admitted at the Department of Stomatology, People’s Hospital of Longhua, Shenzhen were selected. (1) In vitro experiment: Concentrated growth factors were extracted by centrifugation, and human gingival fibroblasts were isolated and cultured. The cells were divided into four groups and cultured in complete culture media containing 0 (control), 25%, 50% and 100% concentrated growth factor extract). (2) In vivo experiment: The affected teeth were extracted followed by dental implantation. Concentrated growth factor was placed to induce the rapid healing of soft and hard tissues. Six months later, two-stage implantation was performed. The modified envelope flap was used to increase the thickness of the labial gingiva and the amount of soft tissue in the proximal and distal areas for 3 months to obtain soft tissue contours in coordination with the adjacent teeth.
RESULTS AND CONCLUSION: (1) In vitro experiment results: Compared with the control group, the proliferation of human gingival fibroblasts was significantly increased by 50% and 100% concentrated growth factor extract on the 3rd day of culture. On the 14th day of culture, compared with the control group, the activity of alkaline phosphatase, the molecular weight of mineralization and the expression of osteoblast-associated genes RUNX2 and osteocalcin were significantly increased in the 100% concentrated growth factor extract group. (2) In vivo experiment results: Concentrated growth factor extract could promote bone regeneration around oral implants. There was no obvious retraction at the gingival margin, and the gingival papilla filled the space of the teeth. The bone mass around the implant increased significantly at 14 months in relative to 3 months after implantation. To conclude, concentrated growth factor extract can promote the proliferation and osteogenic differentiation of human gingival fibroblasts and the peri-implant regeneration of soft and hard tissue.

Key words: concentrated growth factor, concentrated growth factor extract, platelets, human gingival fibroblasts, proliferation, differentiation, oral implantation, tissue construction, soft tissue regeneration, bone tissue regeneration

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