Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (3): 427-434.doi: 10.3969/j.issn.2095-4344.0613

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Different intensities of endurance training affect serum Irisin level and skeletal muscle protein expression of PGC-1alpha, FNDC5, and PPARdelta in mice with obesity induced by a high-fat diet

Su Kunxia   

  1. (Public Education Department of Xin Lian College, Henan Normal University, Zhengzhou 451400, Henan Province, China)
  • Received:2018-07-28
  • About author:Su Kunxia, Master, Lecturer, Public Education Department of Xin Lian College, Henan Normal University, Zhengzhou 451400, Henan Province, China

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Abstract:

BACKGROUND: Peroxisome proliferators-activated receptor-γ coactivator-1 alpha (PGC-1α), fibronectin type III domain-containing protein 5 (FNDC5) and Irisin in mice have been shown to make a positive pathway of lipolysis, and PGC-1α can regulate the expression of peroxisome proliferator-activated receptor delta (PPARδ), which regulates lipolysis in skeletal muscle. Endurance training can effectively reduce the body mass of mice, but the effect of different intensities of endurance training on serum Irisin level, and PGC-1α, FNDC5, PPARδ expression in mice with obesity induced by a high fat diet is still unclear.
OBJECTIVE: To study the effect of high- and low-intensity endurance training on serum Irisin level, and lipolysis-related proteins in mice with obesity induced by a high fat diet.
METHODS: Four-week-old male C57BL/6J mice were fed normal diet for 1 week and then fed 8 weeks of high-fat diet to establish a mouse model of nutritional obesity. The control mice were fed a normal diet. After 1 week of adaptive treadmill training, the obese mice were divided into high- and low-intensity endurance training groups for treadmill training and the control group had no training. After 8 weeks, the body length, body mass and Lee’s index were measured and recorded. The level of Irisin in mouse serum was detected by ELISA. The expression levels of PGC-1α, FNDC5 and PPARδ in quadriceps femoris were detected by western blot assay.
RESULTS AND CONCLUSION: High-fat diet could significantly increase body length, body mass and Lee’s index of mice, suggesting that high-fat-induced nutritional obesity mouse model was established. High-fat diet could significantly reduce the level of Irisin in serum and the expression of lipolysis-related proteins PGC-1α, FNDC5 and PPARδ in quadriceps femoris. Endurance training, especially high-intensity endurance training could effectively reduce the body length, body mass and Lee’s index of mice. Endurance training, especially high-intensity endurance training could effectively increase the level of Irisin in serum and the expression of PGC-1α, FNDC5 and PPARδ in quadriceps femoris. PGC-1α, FNDC5 and Irisin have consistent expression, suggesting that endurance training, especially high-intensity endurance training can up-regulate the expression of skeletal muscle PGC-1α, thereby increasing the expression of FNDC5 protein. FNDC5 is transformed into Irisin and released into the blood, thus increasing the level of lipolysis in mice, and achieving weight loss.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Resistance Training, Muscle, Skeletal, Mice, Obese, Egg White, Tissue Engineering

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