Establishment and identification of a rabbit model of comorbid osteoarthritis and osteoporosis

doi:10.3969/j.issn.2095-4344.1025

Abstract

Abstract:

BACKGROUND: The elderly often suffers from comorbid osteoarthritis and osteoporosis, but the relationship between osteoarthritis and osteoporosis is not yet clear.
OBJECTIVE: To establish and identify an animal model of comorbid osteoarthritis and osteoporosis.
METHODS: Thirty-seven female New Zealand white rabbits at 2 months of age were provided by the Animal Center of Guiyang Medical University, China. Of the 37 rabbits, 30 rabbits were selected and divided into osteoarthritis group, osteoporosis group, osteoarthritis and osteoporosis group. The remaining seven rabbits were used as normal control group. Different animal models of osteoporosis (excision of bilateral ovarian and intramuscular injection of methylprednisolone)/osteoarthritis (excision of anterior cruciate ligament)/osteoarthritis and osteoporosis (excision of bilateral ovaries and anterior cruciate ligament+intramuscular injection of methylprednisolone) were made in corresponding groups. After successful modeling, cartilage samples from each rabbit were isolated and cultured. Secretion of type II collagenase was compared among three groups.
RESULTS AND CONCLUSION: (1) After excision of rabbit anterior cruciate ligament and bilateral ovaries and intramuscular injection of methylprednisolone, the bone density of osteporotic rabbits was reduced by more than 25%. Exfoliation of the articular cartilage could be observed from the joint appearance, and osteophyte formed markedly shown on the X-ray film of the knee joint obviously. Obvious articular cartilage damage was also observed on the MRI image. Therefore, the model of comorbid osteoarthritis and osteoporosis was successfully prepared by the above methods. (2) Chondrocytes could be successfully extracted from the rabbit knee joint cartilage in the three model groups. The ability of chondrocytes secreting type II collagenase was significantly lowered in the osteoporosis group compared to the osteoarthritis and osteoporosis group (P < 0.05). (3) To conclude, the animal model of comorbid osteoarthritis and osteoporosis was successfully established.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoarthritis, Osteoporosis, Models, Animal, Tissue Engineering

CLC Number:

R445

Ma Wenjuan1, Zhang Kaiwei2, Shen Fengjun2, Deng Zhiyuan3, Cheng Xiaojin4. Establishment and identification of a rabbit model of comorbid osteoarthritis and osteoporosis[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(3): 421-426.

Figures/Tables 7

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