Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (3): 372-377.doi: 10.3969/j.issn.2095-4344.0588

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A centrifuge device for high acceleration loading in rats: mechanobiological responses of animals in a high-acceleration environment

Gao Zijian1, Yu Lu2,3, Gao Lilan1, Zhang Chunqiu1, Zhang Xizheng2   

  1.  (1Key Laboratory of Advanced Electromechanical System Design and Intelligent Control, National Experimental Teaching Demonstration Center of Mechatronics Engineering, School of Mechanical Engineering, Tianjin University of Technology, Tianjin 300384, China; 2Institute of Medical Equipment of Academy of Military Medical Sciences, Tianjin 300000, China; 3Institute for Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030000, Shanxi Province, China)
  • Received:2018-07-27 Online:2019-01-28 Published:2019-01-28
  • Contact: Zhang Chunqiu, PhD, Professor, Key Laboratory of Advanced Electromechanical System Design and Intelligent Control, National Experimental Teaching Demonstration Center of Mechatronics Engineering, School of Mechanical Engineering, Tianjin University of Technology, Tianjin 300384, China
  • About author:Gao Zijian, Master candidate, Key Laboratory of Advanced Electromechanical System Design and Intelligent Control, National Experimental Teaching Demonstration Center of Mechatronics Engineering, School of Mechanical Engineering, Tianjin University of Technology, Tianjin 300384, China
  • Supported by:

    the National Natural Science Foundation of China (Key Program), No. 11432016 (to ZXZ); the National Natural Science Foundation of China (General Program), No. 81741141 (to ZCQ) 

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Abstract:

BACKGROUND: Manned spacecraft launches and returns, and shipboard aircraft takes off and landing, will expose astronauts and pilots to high acceleration environment. High acceleration environment has an important impact on cells, organs, and bodies. The high acceleration centrifuge is a device that can provide high acceleration environment for cells and animals. It plays a role of basic platform in the study on mechanics biology and tissue engineering, especially biological laboratory.
OBJECTIVE: To design a centrifuge for high acceleration loading in rats based on a centrifuge and to study the mechanobiological responses of rats in a high-acceleration environment.
METHODS: Design process of centrifuge was as follows: determine parameters and select motor → design and validate of the centrifuge → select controller → manufacture and commission machine. Rats were loaded horizontally and vertically. Four groups including 4 G group, 8 G group, 10 G group and 20 G group and one control group were set up. The effects of different high acceleration environments on the physical condition and walking ability of rats were detected.
RESULTS AND CONCLUSION: We designed and manufactured the centrifuge, and it included two parts: mechanical structure and control system. The machine run smoothly and had no noise, and could provide 0-40 G high acceleration environment and variable acceleration environment. After rats were subjected to different high acceleration loads, their bodies were inclined to one side and could not walk in a straight line, and accompanied by trembling, hindlimb congestion and other symptoms. The higher the high acceleration value, the greater the influence on rats. After a period of rest, the body could basically recover. Our test suggests that the centrifuge can provide a high acceleration environment for animal experiments, which can be used to study the mechanobiological responses of animals in high acceleration environment.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Acceleration, Biomechanics, Centrifugation, Tissue Engineering

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