Simvastatin sustained-release microspheres promote osteogenesis of bone tissue engineering in vitro

doi:10.3969/j.issn.2095-4344.1511

Abstract

Abstract:

BACKGROUND: In recent years, domestic and foreign studies have assembled simvastatin sustained-release microspheres onto scaffold materials. It has been found that simvastatin can be used to repair bone defects and good results have been obtained.

OBJECTIVE: To investigate the effect of simvastatin sustained-release microspheres on tissue engineering osteogenesis.

METHODS: Nanoliposome simvastatin sustained-release microspheres were prepared by thin-film dispersion method. The particle size, polydispersity coefficient and encapsulation efficiency were measured. The sustained release properties of free simvastatin solution and simvastatin sustained-release microsphere solution were measured by dialysis device. The third generation of human placental mesenchymal stem cells was inoculated on porous hydroxyapatite ceramic scaffolds. The cells were cultured in two groups after adherent growth. The experimental group was treated with simvastatin sustained-release microspheres, and the normal medium was added to the blank group. Transcriptome sequencing was carried out after 7, 14, 21 days. The expression levels of osteogenic genes were verified by real-time fluorescence quantitative PCR.

RESULTS AND CONCLUSION: (1) The average particle size of simvastatin sustained-release microspheres was (77.27±6.4) nm, the polydispersity coefficient was 0.131±0.040, and the encapsulation efficiency was 85.6%. (2) The rapid release of free simvastatin solution exceeded 80% total dose in the first 3 days. While the simvastatin sustained-release microsphere solution released only about 40% of the drug in the first 3 days, and had been slowly released until the 14^th day, and the cumulative release at the 14^th day was close to 80%. (3) Transcriptome sequencing showed that the osteogenesis-related genes such as bone morphogenetic protein 2, bone morphogenetic protein 4 and vascular endothelial growth factor were enriched and expressed in the experimental group. (4) Real-time quantitative PCR showed that the mRNA levels of bone morphogenetic protein 2, bone morphogenetic protein 4 and vascular endothelial growth factor were significantly higher in the experimental group than the blank group at 7, 14 and 21 days (P < 0.05). These results indicate that simvastatin sustained-release microspheres can promote osteogenic performance for tissue engineering.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Hydroxyapatites, Liposomes, Tissue Engineering

CLC Number:

R459.9

R318

Qing Wei, Dai Yanjun, Huang Lijuan, Ren Jing, Zheng Jiajun, Tuo Qiang, Ren Xiaohua, Mu Yandong. Simvastatin sustained-release microspheres promote osteogenesis of bone tissue engineering in vitro[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(2): 239-244.

Figures/Tables 7

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