3D bioprinted nozzle for skin scaffolds: CFD simulation and experiments

doi:10.3969/j.issn.2095-4344.1512

Abstract

Abstract:

BACKGROUND: With the development of tissue engineering, 3D bioprinting technology is used to prepare skin scaffolds. The process parameters are however unclear, and stacking and discontinuities often occur during the printing process.

OBJECTIVE: To conduct the numerical simulation by 3D bio-printer and finite element software Fluent so as to obtain reasonable extrusion pressure, viscosity, nozzle walking speed, nozzle diameter and temperature parameters to solve the problem of accumulation and discontinuity in the printing process of gelatin fibers.

METHODS: Using 5% gelatin cellulose solution as the printing material, the numerical simulation was carried out by using 3D bio-printer and software Fluent. The printing temperature was set at 5, 15, 25 ^oC, and the printing pressure was 0.16, 0.18, 0.2 MPa, respectively. The flow rate and flow at the tip of the nozzle were measured at 0.21, 0.26, and 0.41 mm. The gelatin fiber skin bio-scaffold was 3D-printed with appropriate parameters, and the scaffold structure was observed by scanning electron microscopy.

RESULTS AND CONCLUSION: When the printing temperature was set at 15 ^oC, the nozzle walking speed was 30 mm/s, the pressure was 0.18 MPa, and the nozzle diameter was 0.21 mm, the gelatin fiber material could be continuously extruded but not stacked or broken. Under the above printing conditions, the width of printed filament was about 1 400 μm. For the biological scaffold printed under this condition, the row and column distribution of the scaffold was more uniform shown by the scanning electron microscope, and the bonding site between the layers was firmly bonded and not easily deformed. The porosity of the scaffold was about 57%.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Gelatin, Tissue Engineering

CLC Number:

R459.9

R318

Chen Dongdong, Zhang Qi, Zhang Pengfei, Zhou Jiping, Jiang Yani. 3D bioprinted nozzle for skin scaffolds: CFD simulation and experiments[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(2): 245-250.

Figures/Tables 7

References

[1] 何雪峰,熊爱兵.3D打印技术在整形外科的研究及应用进展[J].中国组织工程研究,2017,21(3):428-432.

[2] 汤永喆.微粒皮结合人工真皮构建组织工程皮肤修复创面的初步研究[D].上海:复旦大学,2010.

[3] 郝文丽,张平,吴训伟.组织工程皮肤的现状和展望[J].北京生物医学工程,2016,35(1):94-99.

[4] 金嘉长,王扬,马维虎,等.3D生物打印技术在组织工程支架构建与再生中的应用进展[J].航天医学与医学工程, 2016,29(6): 462-468.

[5] 罗文峰,杨雪香,敖宁建.生物医用材料的3D打印技术与发展[J].材料导报,2016,30(13):81-86.

[6] 石静,钟玉敏.组织工程中3D生物打印技术的应用[J].中国组织工程研究,2014,18(2):271-276.

[7] 胡锦花.生物3D打印多层皮肤组织模型的研究[D].杭州:杭州电子科技大学,2017:1-54.

[8] 史建峰,王涵,王迎,等.3D生物打印技术的发展与应用[J].医疗装备,2017,30(17):202-204.

[9] 王璐,胡为杰,聂昊,等.3D打印与组织工程心肌、心脏瓣膜、大血管及血管网的构建[J].中国组织工程研究, 2015,19(43): 7029-7034.

[10] 房瑞,许零,陈欣,等.组织工程皮肤支架材料和种子细胞的研究进展[J].中国组织工程研究与临床康复,2009,13(47):9329-9333.

[11] 顾奇,郝捷,陆阳杰,等.GordonG.Wallace,周周琪.生物三维打印的研究进展[J].中国科学:生命科学,2015,45(5):439-449.

[12] 杨润怀,陈月明,马长望,等.生物物细胞三维打印技术与材料研究进展[J].生物医学工程学杂志,2017,34(2):320-324.

[13] 孟炯.满足个性化需求的3D打印定制模式创新——兼论实现个性化定制模式的商业化策略[J].科技进步与对策, 2016,33(15): 22-29.

[14] Boland T,Xu T,Damon B,et al.Application of Inkjet Printing to Tissue Engineering.Biotechnol J. 2006;1(9):910.

[15] Xu T,Gregory CA,Molnar P,et al.Viability and electrophysiology of neural cell structures generated by the inkjet printing method.Biomaterials.2006;27(19):3580-3588.

[16] Khalil S,Nam J,Sun W.Multi‐nozzle deposition for construction of 3D biopolymer tissue scscaffolds.Rapid Prototyping J.2005;11(1):9-17.

[17] Michael S,Sorg H,Peck CT,et al.Tissue Engineered Skin Substitutes Created by Laser-Assisted Bioprinting Form Skin-Like Structures in the Dorsal Skin Fold Chamber in Mice.Plos One.2013; 8(3):e57741.

[18] 任荣,张剑飞,司家文,等.海藻酸钠/明胶复合水凝胶用于3D生物打印的初步研究[J].中国口腔颌面外科杂志, 2017,15(5): 402-407.

[19] Defraeye T,Blocken B,Carmeliet J.CFD analysis of convective heat transfer at the surfaces of a cube immersed in a turbulent boundary layer.Int J Heat Mass Transfer. 2010;53 (1-3):297-308. [20] 刘露露,盛永林,童李.基于Fluent自进式旋转射流喷头的数值模拟[J].煤矿机电,2010,31(5):4-5

[21] 田琦,檀盼龙.T型微通道内流体流动的FLFLUENT模拟[J].云南化工,2017,44(7):57-59.

[22] 任翀,闫恪涛,范丽荣.基于FLUENT模拟的熔融沉积快速成型喷头工艺参数研究[J].铸造技术,2017,38(6):1406-1409.

[23] 廉慧珍,李玉琳.关于体积法计算混凝土配合比中原材料密度的问题[J].混凝土,2010,25(9):1-3.

[24] 杨倩倩.分离流量法的非牛顿流体润滑理论、计算与实验研究[D].广州:华南理工大学,2016.

[25] 于洋,宗光华,丁凤林.超声波流量测量中流速计算方法的对比[J].北京航空航天大学学报,2013,39(1):37-41.

[26] 王康建,曾睿,但卫华,等.基于天然高分子材料的组织工程化皮肤支架材料[J].生物医学工程与临床,2009, 13(2):161-166.

[27] 张杨,李秀兰,师宜健.含有生肌液的明胶-壳聚糖皮肤支架的生物学特性研究[J].生物医学工程与临床, 2007,11(2):97-101.

[28] 牛金柱,李丽华,李富文,等.利多卡因明胶纤维止血止痛敷料的研制及临床应用[J].中国医药导报,2008,5(6):97.

[29] 杨宇,仇雄文,郑清健,等.应用带硅胶膜的植入式真皮再生支架修复耳廓的中小面积皮肤缺损[J].中国医疗美容,2017,7(4):17-20.

[30] 王克敏,李世荣,郭嘉.热致相分离技术制备组织工程支架[J].化学与生物工程,2006,23(1):1-3.

[31] 师奇松,于建香,顾克壮,等.静电纺丝技术及其应用[J].化学世界, 2005,46(5):313-316.

[32] 廖俊琳,王少华,陈佳,等.3D生物打印在组织工程软骨再生与重建应用中的研究进展[J].中南大学学报(医学版), 2017,42(2): 221-225.

[33] 陈绍军,叶旋,钟燕辉.3D打印技术在生物医用高分子材料制备领域的应用进展[J].广东化工, 2018,45(4):123-124.

[34] 李大为,何进,何凤利,等.丝素蛋白/壳聚糖复合材料在组织工程中应用的研究进展[J].中国生物工程杂志, 2017,37(10): 111-117.

[35] 牛金柱,牛金亮,刘宿,等.明胶纤维网粘附性及生物吸收性的实验研究[J].解放军医药杂志,2001,13(1):8-9.

[36] 关嫚,任磊,吴婷,等.新型双层皮肤组织工程支架的构建[J].厦门大学学报(自然科学版),2006,45(3):379-382.