Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (34): 5452-5457.doi: 10.3969/j.issn.2095-4344.0680

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Repair of mandibular defects with polyetheretherketone/biphase bioceramic composites

Yu Hedong1, Leng Weidong1, Chen Yongji1, Ni Xiaobing1, Ai Jun1, Tan Yaqin1, Luo Jie2   

  1. 1Department of Stomatology, 2Department of Neurosurgery, Taihe Hospital (Affiliated Hospital, Hubei University of Medicine), Shiyan 442000, Hubei Province, China
  • Received:2018-08-25 Online:2018-12-08 Published:2018-12-08
  • Contact: Luo Jie, Chief physician, Professor, Doctoral supervisor, Department of Neurosurgery, Taihe Hospital (Affiliated Hospital, Hubei University of Medicine), Shiyan 442000, Hubei Province, China
  • About author:Yu Hedong, Master, Attending physician, Department of Stomatology, Taihe Hospital (Affiliated Hospital, Hubei University of Medicine), Shiyan 442000, Hubei Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81671831, 51541202

Abstract:

BACKGROUND: Polyetheretherketone has good affinity and corrosion resistance, and its biocompatibility is stable. Bioceramic materials also have good mechanical properties and bio-affinity. Therefore, polyetherketone can be combined with biphase bioceramics to prepare composite scaffolds that may promote the repair of bone defects. 

OBJECTIVE: To investigate the effect of polyetheretherketone/biphasic bioceramic composites in the repair of rabbit mandibular defects.
METHODS: Twenty New Zealand white rabbits (purchased from the Animal Experimental Center, Hubei University of Medicine, China) were divided into four groups. In the control group, no treatment was given. In the operation group, a mandibular defect model was made in the rabbit. In the composite scaffold group, polyetheretherketone/biphasic bioceramic composite scaffold was implanted into the mandibular defect. In the sham group, the incision was directly sutured after exposing the gingival groove. Mandibular specimens in each group were taken at 4, 8, and 16 weeks after surgery, and hematoxylin-eosin staining, Goldner’s trichrome staining, and bone morphogenetic protein-2 expression were detected.
RESULTS AND CONCLUSION: (1) Results from the hematoxylin-eosin staining and Goldner’s trichrome staining revealed that: in the composite scaffold group, osteoblasts actively grew into the pores at 4 weeks after surgery, and peaked at 16 weeks, while in the operation group, granulation tissues and a small amount of osteoblasts were visible, but the number of osteoblasts was lower relative to the composite scaffold group. (2) PCR results showed that the mRNA expression of bone morphogenetic protein 2 was significantly higher in the composite scaffold group than the control and operation groups at 8 and 16 weeks after surgery (P < 0.05 or P < 0.01). (3) Western blot results indicated that the protein expression of bone morphogenetic protein 2 was significantly higher in the composite scaffold group than the control and operation groups at 4, 8, 16 weeks after surgery (P < 0.05 or P < 0.01). To conclude, the polyetheretherketone/biphasic bioceramic composite scaffold can effectively promote the expression of bone morphogenetic protein 2 in the cells, and then promote the growth and differentiation of osteoblasts, while effectively repairing bone defects.  

Key words: Bone Morphogenetic Proteins, Mandible, Tissue Engineering

CLC Number: