Effect of baicalin on human periodontal ligament fibroblasts and the underlying molecular mechanism

doi:10.3969/j.issn.2095-4344.0390

Abstract

Abstract:

BACKGROUND: Baicalin, as an important traditional Chinese medicine extract, has been extensively studied. Baicalin has been shown to promote the proliferation of human periodontal ligament fibroblasts (HPLFs) and differentiation into osteocytes, but the underlying molecular mechanism remains unclear.
OBJECTIVE: To study the effect of baicalin on the proliferation of HPLFs and the underlying molecular mechanism.
METHODS: Morphology of HPLFs was observed by primary cell culture and light microscopy. The growth-promoting effect of baicalin on HPLFs was detected by cell counting kit-8 assay. The effect of baicalin on the expression of proliferating cell nuclear antigen was detected by qRT-PCR. The inhibitory effect of baicalin on cell apoptosis was detected by qRT-PCR and flow cytometry. The level of bone morphogenetic protein 2 (BMP2) under baicalin treatment was tested by western blot assay and qRT-PCR. BMP2 antibody was employed to detect the influence of baicalin on the proliferation and apoptosis of HPLFs and the expression of BMP2 in HPLFs.
RESULTS AND CONCLUSION: Compared with the control group, baicalin could significantly promote the proliferation of HPLFs, and up-regulate the mRNA expression level of proliferating cell nuclear antigen. Compared with the control group, baicalin could significantly up-regulate the mRNA level of Bcl-2 and down-regulate the mRNA level of Bad. Flow cytometry also showed that baicalin could inhibit cell apoptosis. Baicalin could up-regulate the protein and mRNA levels of BMP2 in a time- and concentration-dependent manner. The regulatory effect of baicalin was decreased after addition of BMP2 antibody. In summary, baicalin promotes proliferation of HPLFs probably via up-regulating BMP2 level.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Drugs, Chinese Herbal, Periodontal Ligament, Cell Proliferation, Bone Morphogenetic Proteins, Tissue Engineering

CLC Number:

R318

Li Li-hua1, Jia Ai-min2, Yang Ming-hui2, Liu Zhen1, Wang Li-heng2, Jiang Hong2, Qiu Ya2. Effect of baicalin on human periodontal ligament fibroblasts and the underlying molecular mechanism[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(28): 4475-4480.

Figures/Tables 3

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