Recombinant human bone morphogenetic protein-2/fibrin gel enhances bone formation in a rabbit model of tibial distraction osteogenesis

doi:10.3969/j.issn.2095-4344.0976

Abstract

Abstract:

BACKGROUND: Numerous reports have been made on the use of bone morphogenetic protein-2 suspended in sustained release carrier in the fracture healing and bone defect repair. However, little is reported on its effect during distraction osteogenesis.

OBJECTIVE: To investigate the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) suspended in fibrin gel on bone formation during distraction osteogenesis in rabbits.

METHODS: The in vitro release kinetics of rhBMP-2 suspended in fibrin gel was tested using an enzyme-linked immunosorbent assay. Unilateral tibial lengthening 10 mm was achieved in 48 healthy New Zealand rabbits. After the completion of osteodistraction, vehicle, fibrin, rhBMP-2 or rhBMP-2 suspended in fibrin (rhBMP-2+fibrin) was injected into the center of lengthened gap in the corresponding group with 12 animals in each group. At 8 weeks after injection, distracted callus was examined by histological, Micro-CT and biomechanical tests. Radiographs of the distracted tibia were taken at both 4 and 8 weeks after injection.

RESULTS AND CONCLUSION: Fibrin gel prolonged the life span of rhBMP-2 in vitro with a sustained release during 17 days. The rabbits undergoing treatment with rhBMP-2+fibrin gel showed the best results in radiation density and cortical bone connection by the radiological observation, in bone mineral density, bone volume fraction, and cortical bone thickness by the Micro-CT evaluation and in ultimate load by the biomechanical test when compared to other groups (P < 0.05). In histological images, rhBMP-2+fibrin treatment showed increased callus formation and better gap bridging compared to the other groups. To conclude, fibrin gel holds the promise to be a good carrier of rhBMP-2, and rhBMP-2 suspended in fibrin promotes bone formation in the rabbit model of tibial distraction osteogenesis.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Bone Morphogenetic Proteins, Fibrin, Gels, Osteogenesis, Tissue Engineering

CLC Number:

R318

Guo Jiao-yang, Xu Lu-chen, Li Yun-feng. Recombinant human bone morphogenetic protein-2/fibrin gel enhances bone formation in a rabbit model of tibial distraction osteogenesis[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(30): 4763-4768.

Figures/Tables 7

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