Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (25): 4077-4082.doi: 10.3969/j.issn.2095-4344.0924
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Feng Peng-fei, Li Xiao-na, Rong Shuo, Chen Wei-yi, Wang Xiao-jun
Revised:
2018-04-21
Online:
2018-09-08
Published:
2018-09-08
Contact:
Chen Wei-yi, Doctoral supervisor, Key Laboratory of Material Intensity and Structural Impact in Shanxi Province, Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, Shanxi Province, China
About author:
Feng Peng-fei, Key Laboratory of Material Intensity and Structural Impact in Shanxi Province, Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, Shanxi Province, China
Supported by:
the National Natural Science Foundation of China, No. 11572213, 31271005, 11402161, 11402162; the Graduate Student Education Innovation Project in Shanxi Province, No. 2015BY28
CLC Number:
Feng Peng-fei, Li Xiao-na, Rong Shuo, Chen Wei-yi, Wang Xiao-jun. Combined effects of interleukin 1beta and mechanical stretching on Collagen I synthesis and Lumican expression in corneal fibroblasts[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(25): 4077-4082.
2.1 白细胞介素1β与机械牵拉对角膜成纤维细胞CollagenⅠα1的影响 白细胞介素1β与机械牵拉共同作用于角膜成纤维细胞,实时荧光定量PCR检测CollagenⅠα1 mRNA的表达,见图1。 未添加白细胞介素1β时,与静态对照组相比,5%,10%,15%分别牵拉12 h使CollagenⅠα1表达下降,差异有显著性意义(P < 0.05);牵拉24 h,CollagenⅠα1的表达在5%牵拉时表达升高,在10%,15%牵拉时表达下降,差异有显著性意义(P < 0.05);牵拉36 h,CollagenⅠα1的表达在5%牵拉时表达升高,在10%,15%牵拉时表达下降,差异有显著性意义(P < 0.05)。 在白细胞介素1β为0.2 μg/L和0.4 μg/L时,与静态对照组相比,CollagenⅠα1的表达在5%和10%牵拉12 h时没有变化,而在15%牵拉12 h时降低,差异有显著性意义(P < 0.05);与静态对照组相比,CollagenⅠα1的表达在5%,10%,15%牵拉24 h和36 h下降,差异有显著性意义(P < 0.05)。 在白细胞介素1β为0.8 μg/L时,与静态对照组相比,CollagenⅠα1的表达在5%,10%,15%牵拉12 h时没有变化,而在牵拉24 h和36 h时降低,差异有显著性意义(P < 0.05)。 相比于未添加白细胞介素1β时,0.2,0.4,0.8 μg/L白细胞介素1β单独作用12 h角膜成纤维细胞CollagenⅠα1的表达显著性降低(P < 0.05)。 2.2 白细胞介素1β与机械牵拉对角膜成纤维细胞CollagenⅠα2的影响 白细胞介素1β与机械牵拉共同作用于角膜成纤维细胞,实时荧光定量PCR检测CollagenⅠα2 mRNA的表达,见图2。 未添加白细胞介素1β时,与静态对照组相比,5%,10%,15%分别牵拉12 h使CollagenⅠα2表达降低,差异有显著性意义(P < 0.05);牵拉24 h和36 h时,CollagenⅠα2的表达在5%牵拉时没有变化,在10%,15%牵拉时表达下降,差异有显著性意义(P < 0.05)。 在白细胞介素1β为0.2,0.4 μg/L时,与静态对照组相比,CollagenⅠα2的表达在5%牵拉12 h时没有变化,而在10%,15%牵拉12 h时降低,差异有显著性意义(P < 0.05);与静态对照组相比,CollagenⅠα2的表达在5%,10%,15%牵拉24 h和36 h下降,差异有显著性意义(P < 0.05)。 在白细胞介素1β为0.8 μg/L时,与静态对照组相比,CollagenⅠα2的表达在5%,10%牵拉12 h时没有变化,而在15%牵拉12 h时降低,差异有显著性意义(P < 0.05);与静态对照组相比,CollagenⅠα2的表达在5%,10%,15%牵拉24 h和36 h下降,差异有显著性意义(P < 0.05)。 2.3 白细胞介素1β与机械牵拉对角膜成纤维细胞Lumican的影响 白细胞介素1β与机械牵拉共同作用于角膜成纤维细胞,实时荧光定量PCR检测Lumican mRNA的表达,见图3。 未添加白细胞介素1β时,与静态对照组相比,5%牵拉12,24,36 h使Lumican表达升高,差异有显著性意义(P < 0.05);10%和15%牵拉12 h使Lumican表达显著性降低(P < 0.05),而牵拉24 h和36 h使Lumican表达升高,差异有显著性意义(P < 0.05)。 在白细胞介素1β为0.2 μg/L时,5%牵拉12,24,36 h使角膜成纤维细胞Lumican的表达升高,差异有显著性意义(P < 0.05);10%和15%牵拉在12 h使角膜成纤维细胞Lumican的表达显著性降低,而在24 h和36 h使Lumican的表达升高,差异有显著性意义(P < 0.05)。 在白细胞介素1β为0.4 μg/L时,5%和10%牵拉12 h使角膜成纤维细胞Lumican的表达没有变化,15%牵拉使Lumican的表达降低,差异有显著性意义(P < 0.05);5%,10%,15%牵拉24 h和36 h使Lumican的表达升高,差异有显著性意义(P < 0.05)。 在白细胞介素1β为0.8 μg/L时,5%,10%,15%牵拉12 h使角膜成纤维细胞Lumican的表达没有变化,5%,10%,15%牵拉24 h和36 h使Lumican的表达升高,差异有显著性意义(P < 0.05)。 当白细胞介素1β单独作用时,与0 μg/L时相比,Lumican在12 h和36 h的表达不随白细胞介素1β的质量浓度而变化;单独作用24 h,在白细胞介素1β质量浓度为0.2,0.4 μg/L时,Lumican的表达升高,差异有显著性意义(P < 0.05)。"
[1] Meek KM, Knupp C. Corneal structure and transparency. Prog Retin Eye Res. 2015;49:1-16.[2] Genina EA, Bashkatov AN, Kamenskikh ID, et al. OCT/LCT monitoring of drug action on the structure of the human cornea in vivo. Journal of Biomedical Photonics & Engineering. 2015; 1(1): 77-80.[3] 邓珍,袁满红. 角膜胶原的研究进展[J].现代生物医学进展, 2006,6(11): 123-124,130.[4] Deng LH, Li WL. Research advances of cornea collagen protein. International Journal of Ophthalmology. 2008; 8(3):557-560.[5] Dai Y, Chen J, Li H, et al. Characterizing the effects of VPA, VC and RCCS on rabbit keratocytes onto decellularized bovine cornea. PLoS One. 2012;7(11):e50114.[6] Yan J, Qiang L, Gao Y, et al. Effect of fiber alignment in electrospun scaffolds on keratocytes and corneal epithelial cells behavior. J Biomed Mater Res A. 2012;100(2):527-535.[7] Jester JV, Ho-Chang J. Modulation of cultured corneal keratocyte phenotype by growth factors/cytokines control in vitro contractility and extracellular matrix contraction. Exp Eye Res. 2003;77(5):581-592.[8] Takács L, Tóth E, Berta A, et al. Stem cells of the adult cornea: from cytometric markers to therapeutic applications. Cytometry A. 2009;75(1):54-66.[9] Shao H, Scott SG, Nakata C, et al. Extracellular matrix protein lumican promotes clearance and resolution of Pseudomonas aeruginosa keratitis in a mouse model. PLoS One. 2013;8(1): e54765.[10] Brézillon S, Pietraszek K, Maquart FX, et al. Lumican effects in the control of tumour progression and their links with metalloproteinases and integrins. FEBS J. 2013;280(10): 2369-2381.[11] Dupps WJ Jr, Wilson SE. Biomechanics and wound healing in the cornea. Exp Eye Res. 2006;83(4):709-720.[12] Caprioli J, Coleman AL. Blood pressure, perfusion pressure, and glaucoma. Am J Ophthalmol. 2010;149(5):704-712.[13] Buys YM, Alasbali T, Jin YP, et al. Effect of sleeping in a head-up position on intraocular pressure in patients with glaucoma. Ophthalmology. 2010;117(7):1348-1351.[14] Lee TE, Yoo C, Kim YY. Effects of different sleeping postures on intraocular pressure and ocular perfusion pressure in healthy young subjects. Ophthalmology. 2013;120(8): 1565-1570.[15] McMonnies CW. Management of chronic habits of abnormal eye rubbing. Cont Lens Anterior Eye. 2008;31(2):95-102.[16] Tseng HC, Lee IT, Lin CC, et al. IL-1β promotes corneal epithelial cell migration by increasing MMP-9 expression through NF-κB- and AP-1-dependent pathways. PLoS One. 2013;8(3):e57955.[17] Okanobo A, Chauhan SK, Dastjerdi MH, et al. Efficacy of topical blockade of interleukin-1 in experimental dry eye disease. Am J Ophthalmol. 2012;154(1):63-71.[18] Chen YT, Nikulina K, Lazarev S, et al. Interleukin-1 as a phenotypic immunomodulator in keratinizing squamous metaplasia of the ocular surface in Sjögren's syndrome. Am J Pathol. 2010;177(3):1333-1343.[19] Wilson SE, Esposito A. Focus on molecules: interleukin-1: a master regulator of the corneal response to injury. Exp Eye Res. 2009;89(2):124-125.[20] Battat L, Macri A, Dursun D, et al. Effects of laser in situ keratomileusis on tear production, clearance, and the ocular surface. Ophthalmology. 2001;108(7):1230-1235.[21] Toda I, Asano-Kato N, Komai-Hori Y, et al. Dry eye after laser in situ keratomileusis. Am J Ophthalmol. 2001;132(1):1-7.[22] Knorz MC. Complications of refractive excimer laser surgery。 Ophthalmologe. 2006;103(3):192-198.[23] Massingale ML, Li X, Vallabhajosyula M, et al. Analysis of inflammatory cytokines in the tears of dry eye patients. Cornea. 2009;28(9):1023-1027.[24] Grosskreutz CL, Hockey HU, Serra D, et al. Dry Eye Signs and Symptoms Persist During Systemic Neutralization of IL-1β by Canakinumab or IL-17A by Secukinumab. Cornea. 2015;34(12):1551-1556.[25] O'Brart DP. Corneal collagen cross-linking: a review. J Optom. 2014;7(3):113-124.[26] Said A, Hamade IH, Tabbara KF. Late onset corneal ectasia after LASIK surgery. Saudi J Ophthalmol. 2011;25(3): 225-230.[27] Shelton L, Rada JS. Effects of cyclic mechanical stretch on extracellular matrix synthesis by human scleral fibroblasts. Exp Eye Res. 2007;84(2):314-322.[28] Archambault J, Tsuzaki M, Herzog W, et al. Stretch and interleukin-1beta induce matrix metalloproteinases in rabbit tendon cells in vitro. J Orthop Res. 2002;20(1):36-39.[29] Oya K, Sakamoto N, Ohashi T, et al. Combined stimulation with cyclic stretching and hypoxia increases production of matrix metalloproteinase-9 and cytokines by macrophages. Biochem Biophys Res Commun. 2011;412(4):678-682.[30] Pierscionek BK, Asejczyk-Widlicka M, Schachar RA. The effect of changing intraocular pressure on the corneal and scleral curvatures in the fresh porcine eye. Br J Ophthalmol. 2007;91(6):801-803.[31] Asejczyk-Widlicka M, ?ródka W, Schachar RA, et al. Material properties of the cornea and sclera: a modelling approach to test experimental analysis. J Biomech. 2011;44(3):543-546.[32] Lu Y, Fukuda K, Seki K, et al. Inhibition by triptolide of IL-1-induced collagen degradation by corneal fibroblasts. Invest Ophthalmol Vis Sci. 2003;44(12):5082-5088.[33] Kiga N, Tojyo I, Matsumoto T, et al. Expression of lumican and fibromodulin following interleukin-1 beta stimulation of disc cells of the human temporomandibular joint. Eur J Histochem. 2011;55(2):e11.[34] Carver W, Nagpal ML, Nachtigal M, et al. Collagen expression in mechanically stimulated cardiac fibroblasts. Circ Res. 1991; 69(1):116-122.[35] Liu J, Yu W, Liu Y, et al. Mechanical stretching stimulates collagen synthesis via down-regulating SO2/AAT1 pathway. Sci Rep. 2016;6:21112.[36] Nemoto T, Kajiya H, Tsuzuki T, et al. Differential induction of collagens by mechanical stress in human periodontal ligament cells. Arch Oral Biol. 2010;55(12):981-987.[37] Sawaguchi N, Majima T, Funakoshi T, et al. Effect of cyclic three-dimensional strain on cell proliferation and collagen synthesis of fibroblast-seeded chitosan-hyaluronan hybrid polymer fiber. J Orthop Sci. 2010;15(4):569-577.[38] Quantock AJ, Meek KM, Chakravarti S. An x-ray diffraction investigation of corneal structure in lumican-deficient mice. Invest Ophthalmol Vis Sci. 2001;42(8):1750-1756.[39] Saika S, Shiraishi A, Liu CY, et al. Role of lumican in the corneal epithelium during wound healing. J Biol Chem. 2000;275(4):2607-2612. |
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