Effect of calcium phosphate coatings on corrosion resistance and biocompatibility of magnesium alloy scaffolds

doi:10.3969/j.issn.2095-4344.0914

Abstract

Abstract:

BACKGROUND: Magnesium alloy has better biocompatibility and can accelerate growth of osteoblasts, but its clinical use is limited because of a high corrosion rate in the physiological environment.

OBJECTIVE: To explore the effect of calcium phosphate coating (Ca-P coating) on corrosion resistance and biocompatibility of AZ31 magnesium alloy scaffolds.

METHODS: Using chemical vapor deposition, Ca-P coating was prepared on the surface of AZ31 magnesium alloy scaffolds. (1) Immersion test in static state: AZ31 magnesium alloy scaffolds with or without Ca-P coating were independently immersed in the DMEM medium and the material corrosion rate of each scaffold was tested at 10, 20 and 30 days after immersion. (2) Cytotoxicity test: MC3T3-E1 cells were cultured in the extracts of AZ31 magnesium alloy scaffolds with 100%, 75%, 50% and 25% Ca-P coatings, and cell proliferation and toxicity grade were determined at 1, 3, 5 days after culture. (3) Cell adhesion assay: MC3T3-E1 cells were separately seeded onto the surface of AZ31 magnesium alloy scaffolds with or without Ca-P coating, and cell number was counted at 6, 12 and 24 hours after culture. (4) Cell proliferation test: MC3T3-E1 cells were separately seeded onto the surface of AZ31 magnesium alloy scaffolds with or without Ca-P coating, and cell number was counted at 1, 3, 5 days after culture. (5) Aggregation test of blood cells: AZ31 magnesium alloy scaffolds with or without Ca-P coating were immersed in anticoagulant rabbit blood samples, and amounts of red blood cells, white blood cells and platelets were determined at 8 minutes after immersion.

RESULTS AND CONCLUSION: (1) The corrosion and toxicity rate of AZ31 magnesium alloy scaffold with Ca-P coating was lower than that without Ca-P coating (P < 0.05) without coatings. (2) Cell toxicity of AZ31 magnesium alloy scaffolds with Ca-P coatings of different concentrations was grade 0 to 1. (3) The number of cells adherent to the surface of AZ31 magnesium alloy scaffolds with Ca-P coating was significantly higher than that without Ca-P coating at 12 and 24 hours after inoculation (P < 0.05). (4) The number of cells cultured in the extract of AZ31 magnesium alloy scaffolds with Ca-P coating was significantly higher than that cultured in the extract of the scaffold without Ca-P coating at 3 and 5 days after culture (P < 0.05). (5) The number of red blood cells, white blood cells and platelets in the scaffolds with or without Ca-P coating has no difference (P < 0.05). To conclude, Ca-P coating not only slows the corrosion rate of AZ31 magnesium alloy scaffolds, but also improves the scaffold biocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Materials Testing, Corrosion, Cell Adhesion, Cell Proliferation, Tissue Engineering

CLC Number:

R318

Ma Fei, Li Xiang, Xie Rui-min, Wang Yong-ping, He Yao-hua. Effect of calcium phosphate coatings on corrosion resistance and biocompatibility of magnesium alloy scaffolds[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(26): 4184-4190.

Figures/Tables 9

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