Preparation and performance evaluation of a biomimetic scaffold derived from the extracellular matrix of a native growth plate

doi:10.3969/j.issn.2095-4344.0782

Abstract

Abstract:

BACKGROUND: The extracellular matrix from the growth plate contains abundant sources of collagens, proteoglycan and signaling molecules. These components and characteristic are very similar to the natural growth plate tissue, and therefore, it is an ideal material to construct tissue-engineered growth plate.

OBJECTIVE: To prepare the columnar biomimetic scaffold using native growth plate extracellular matrix and to evaluate the scaffold performance.

METHODS: The knee joint of fetal bovine was separated and purified by pulverization technology in moist environment, differential centrifugation and organic solvent to obtain extracellular matrix microfilaments. Then the extracellular matrix microfilaments were treated by directed crystallization technology, freeze drying and physicochemistry crosslinking method in order to prepare the extracellular matrix biomimetic scaffold. The scaffold was evaluated by scanning electron microscope for internal structure and pore size, porosity and swelling rate of water absorption as well as by histological observation.

RESULTS AND CONCLUSION: The extracellular matrix microfilaments were fibriform with the even and regular shape shown by hematoxylin-eosin staining, and were also positive for toluidine blue, Sarranine O and collagen II staining, indicating the existence of active components of the growth plate in the scaffold. (2) The scaffold under the scanning electron microscope showed porous honeycomb structure on the transverse section plane with uniform pore size and regular shape, while there was a parallel arrangement of columnar structure on the longitudinal section plane of the scaffold with well-distributed and mutual communication, and scaffold fibers on the outer wall were longitudinally arranged, which imitated the columnar structure from the native growth plate. The pore diameter of scaffold was (117.5±15.37) μm, the porosity was (92.05±1.54)% and the water absorption was (95.95±1.07)%. To conclude, the columnar scaffold from the extracellular matrix of the growth plate imitates the native growth plate in terms of components and structures.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Cartilage, Extracellular Matrix;, Growth Plate, Tissue Engineering

CLC Number:

R318

Li Wen-chao, Xu Rui-jiang, Huang Jing-xiang, Li Hao-yu, Cai Gang, Chen Hui. Preparation and performance evaluation of a biomimetic scaffold derived from the extracellular matrix of a native growth plate[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.0782.

Figures/Tables 3

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