Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (36): 6790-6794.doi: 10.3969/j.issn.2095-4344.2012.36.025

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Expression of microRNA-128b, -181 a and -223 in patients with acute leukemia

Tan San-qin1,2, Wang Guang-ping1, Cui Ya-juan1, Xie Yi3, Tan Yu-ting2, Chen Fang-ping1   

  1. 1Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China;
    2Medical College of Hunan Normal University, Changsha 410013, Hunan Province, China;
    3Department of Oncology, Changsha Central Hospital, Changsha 410004, Hunan Province, China
  • Received:2011-12-01 Revised:2011-12-16 Online:2012-09-02 Published:2012-09-02
  • Contact: Chen Fang-ping, Master, Professor, Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China xychenfp@2118.cn
  • About author:Tan San-qin☆, M.D., Associate professor, Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China; Medical College of Hunan Normal University, Changsha 410013, Hunan Province, China tsqsghtz@yahoo.com.cn

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Abstract:

BACKGROUND: MicroRNA (miR) is a kind of endogenous non-coding RNA. Current studies have demonstrated that miR can be used as tumor marker for tumor categorization.
OBJECTIVE: To investigate the expressions of microRNA-181a, miR-128b and miR-223 and their significance in the plasma of patients with acute leukemia.
METHODS: The plasma RNAs were extracted from acute leukemia patients and normal control subjects, and were reverse transcribed with miR-specific primer into cDNA. The expressions of miR-181a, miR-128b and miR-223 were measured by real-time PCR.
RESULTS AND CONCLUSION: Compared with control subjects, the expression of miR-181a, miR-128b and miR-223 was decreased in acute non-lymphocytic leukemia and acute lymphoblastic leukemia patients. Statistical analysis indicated that the expression of miR-128b and miR-181 was significantly different between acute non-lymphocytic leukemia and acute leukemia patients and control subjects (P < 0.05), but its expression was not significantly different between acute lymphoblastic leukemia patients and conntrol subjects (P > 0.05). Compared with acute lymphoblastic leukemia patients, the expression of miR-223 was increased in acute non-lymphocytic leukemia patients, while miR-128b and miR-181a expressions were decreased. The expression of miR-181a, miR-128b and miR-223 was not significantly different between acute non-lymphocytic leukemia and acute lymphoblastic leukemia patients (P > 0.05). These findings suggest that miR-128b, miR-181a and miR-223 may be the novel biomarkers for the diagnosis of typing of acute leukemia.

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