Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (10): 1499-1505.doi: 10.3969/j.issn.2095-4344.0815
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Received:
2017-11-23
Online:
2018-04-08
Published:
2018-04-08
Contact:
Li Guang-da, Ph.D., Associate professor, Master’s supervisor, College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471023, Henan Province, China
About author:
Li Xiao-yu, Master, Central Lab, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China College of Medical; Technology and Engineering, Henan University of Science and Technology, Luoyang 471023, Henan Province, China
Supported by:
CLC Number:
Li Xiao-yu, Li Guang-da, Zhao San-tuan, Li Yong-kai, Zhao Peng-chao, Peng Huan. Preparation and characterization of paeonol loaded brushite calcium phosphate cement[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(10): 1499-1505.
2.1 复合骨水泥的固化时间、可注射系数和物相组成 图1为骨水泥固化时间及可注射系数,从中可见,随丹皮酚含量增加,骨水泥固化时间延长,从0%试样的13 min延长到5%试样的17 min,但均在20 min以内,符合临床使用要求[24]。骨水泥的可注射系数均在95%左右,可见加入丹皮酚未对骨水泥可注射性造成显著影响。透钙磷石骨水泥固相粉末在水化时会发生如下化学反应生成透钙磷石[13]: 在试样的X射线衍射仪谱图中,在2θ约12°、21°、29.2°、30.5°、34.1°和34.4°等处观察到透钙磷石(PDF#09-0077)典型的强衍射峰,说明骨水泥反应充分,丹皮酚的加入未对材料晶相产生显著影响(图2)。各试样的红外测试结果相似,均在3 540,3 485 cm-1和3 289,3 165 cm-1处出现结晶水O-H的吸收峰,在1 651 cm-1处出现H-O-H的吸收峰,并在1 140,1 066和990 cm-1处观察到PO4基团的吸收峰[25](图3)。 从图4可以看出,各试样超微结构存在明显差异。未加入丹皮酚的样品表面为纵横交错的片状结晶,大小均匀,由于表面应力取向及视觉原因,呈针状。随着丹皮酚含量增加,结晶体逐渐变大、增厚,且形态不规则。"
2.3 复合骨水泥的可降解性能及药物缓释性能 骨水泥材料在植入体内后,需要最终全部降解并且降解速率与骨组织新生速率相匹配,而透钙磷石骨水泥可以在体内全部降解[26]。图7为骨水泥试样浸泡7 d和14 d后的失重率。浸泡7 d的试样失重率从0%试样的2.51%增加到5%试样的7.56%,而浸泡14 d的试样的失重率从0%试样的7.71%增加到5%试样的12.77%。 随着骨水泥试样中丹皮酚含量的增加,试样的降解速度加快,并且浸泡14 d的试样的失重率明显高于7 d组。 图8为骨水泥中丹皮酚的单日释放量拟合曲线,可见载丹皮酚透钙磷石骨水泥样品浸泡入介质溶液后,溶液中药物浓度逐渐上升,第3天时,丹皮酚单日释放量达到峰值,而后药物溶出逐渐稳定。 "
2.4 复合骨水泥的抗菌性能表征与细胞亲和性能 从图9可看出,在培养18 h后,不负载丹皮酚的骨水泥对大肠杆菌和金色葡萄球菌的生长都不具有抑制作用。而随着丹皮酚含量的增加,骨水泥对大肠杆菌的抑制作用逐渐加强,1%、3%和5%试样的抑菌圈大小分别提高到约6 mm、8 mm和 10 mm。但各试样对金色葡萄球菌却未显示出抑制生长能力。 图10为材料与细胞复合培养3 d后的材料表面微观形貌,可见在浸泡入培养基中一段时间后,各骨水泥试样的表面形貌发生了显著变化,由以前的紧密排列片状晶粒转换成了许多疏松的细小球状颗粒。众所周知,透钙磷石骨水泥降解速度较快,细胞在其上黏附性差,较难在体外观察到材料上有细胞黏附,但实验中在材料与细胞共同培养3 d后可见细胞在各材料表面的黏附。"
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