A novel vitrified cryopreservation method for the establishment of tumor tissue biobank

doi:10.3969/j.issn.2095-4344.0095

Abstract

Abstract:

BACKGROUND: Vitrified cryopreservation is a novel method for specimen preservation, which has a potential application value in the establishment of tumor biopsy biobank.
OBJECTIVE: To investigate the effectiveness of vitrified cryopreservation and the feasibility in the establishment of tumor biopsy biobank.
METHODS: The fresh biopsy specimens of liver metastasis from rectal cancer were randomized into vitrified cryopreservation and control groups. Then, the biopsy specimens from each group were implanted subcutaneously into the mouse back to establish the humanized mouse xenograft model. The biological characteristics and histological changes of tumor tissues in each group were detected by Calcein-AM/Hoechst33342 staining, as well as immunohistochemistry and hematoxylin-eosin staining. Totally 105 biopsy tissues of liver metastasis from rectal cancer were preliminarily collected and cryopreserved to establish the tumor biopsy biobank.
RESULTS AND CONCLUSION: No significant differences in biological viability or histological features of tumor biopsy was detected before and after cryopreservation (P > 0.05), suggesting that vitrified cryopreservation can effectively preserve tumor biopsy and is favorable for the establishment of tumor biopsy biobank.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Cryopreservation, Liver Neoplasms, Tissue Engineering

CLC Number:

R318

Zhang Yuan, Yang Qiu-rui, Zhang Hong-dan, Zhu Xue-jing, Li Wei-jian, Wang Zhen-yu, Jing Hong-shu, Zhai Bo. A novel vitrified cryopreservation method for the establishment of tumor tissue biobank[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(4): 587-592.

Figures/Tables 1

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