Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (36): 6663-6668.doi: 10.3969/j.issn.2095-4344.2012.36.003

Previous Articles     Next Articles

Culture of bone marrow meenchymal stem cells from a rabbit model of steroid-induced avascular necrosis of the femoral head

Zheng Song-wen1, Wang Nan1, Wang Yan2, Liu Yang2, Jiang Yuan1, Dong Yue1   

  1. 1Department of Radiology, 2Sino-UK Regenerative Medicine Center, First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China
  • Received:2012-02-01 Revised:2012-03-19 Online:2012-09-02 Published:2012-09-02
  • Contact: Dong Yue, M.D., Associate professor, Department of Radiology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China dyy1026@sina.com
  • About author:Dong Yue, M.D., Associate professor, Department of Radiology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China dyy1026@sina.com

PDF

318

Abstract:

BACKGROUND: Autologous stem cell transplantation has become the main emphasis in tissue engineering and regenerative medicine research. In vitro culture of bone marrow mesenchymal stem cells for autologous bone marrow transplantation in a rabbit model of steroid-induced avascular necrosis of femoral head has been rarely reported.
OBJECTIVE: Bone marrow mesenchymal stem cells from a rabbit model of steroid-induced avascular necrosis of femoral head were isolated for in vitro proliferation and phenotype identification.
METHODS: Ten adult big-ear rabbits were divided into two groups. In the control group, two rabbits received no drugs. In the model group, eight rabbits were prepared into model of steroid-induced avascular necrosis of femoral head by intravascular administration of 8 mg/kg methylprednisolone twice a week. Bone marrow mesenchymal stem cells were in vitro isolated and cultured. Surface antigen CD29, CD34 and CD44 expression of passage 4 bone marrow mesenchymal stem cells was detected by flow cytometry.
RESULTS AND CONCLUSION: MR perfusion-weighted imaging and pathological sections results showed that after treatment with a large amount of steroid, rabbit models of steroid-induced avascular necrosis of femoral head had been successfully prepared. In the model group, within 4 hours after steroid application, only a few primary bone marrow mesenchymal stem cells adhered to the culture flask wall, and at 12 days, cells covered 85%-90%of flask bottom; passage 4 bone marrow mesenchymal stem cells grew in a typical “S” shape, and they reached a peak growth pattern, which was similar to the growth curve of the control group. Flow cytometry showed that passage 4 bone marrow mesenchymal stem cells were negative for CD34 expression and positive for CD29 and CD44 expression, indicating that the cultured cells were very pure bone marrow mesenchymal stem cells. These findings suggest that bone marrow mesenchymal stem cells of rabbit models of steroid-induced avascular necrosis of femoral head show strong growing and proliferative capability and can be used for stem cells labeling and further autologous transplantation therapy.

CLC Number: