Germline degradation of a mouse model of familial amyotrophic lateral sclerosis when breeding

doi:10.3969/j.issn.2095-4344.2013.24.022

Abstract

Abstract:

BACKGROUND: SOD1-G93A transgenic mouse is a classical morbid animal model in the investigation of amyotrophic lateral sclerosis. However, there are few reports about the germline deterioration of this transgenic mouse model when breeding.
OBJECTIVE: To investigate the germline deterioration of mouse model of familial amyotrophic lateral sclerosis when breeding.
METHODS: The times of pregnancy, litter size among different generations and the ratios of male and positive of the filial generation were compared to analyze the change of fertility of amyotrophic lateral sclerosis mouse model of SOD1-G93A transgenic mouse when breeding. And the times of invasion and death were compared to analyze the change of pathogenesis of this transgenic mouse when breeding.
RESULTS AND CONCLUSION: The times of pregnancy, per litter size and per mice were decreased significantly (P < 0.05) when comparing the third and sixth generation with the first generation, and moreover there were over half of the mouse of the sixth generation who can not inbreed. The ratios of male and positive mouse of the filial generation also decreased, but the difference was not significant. The time of invasion and death delayed significantly by 6.37 days (P=0.004) and 9.67 days (P=0.022) respectively when comparing the sixth generation with the first generation, and the difference was significant. As a result, familial amyotrophic lateral sclerosis mouse showed deteriorated germline when breeding such as the fertility decline and the delay of the time of invasion and death.

Key words: tissue construction, basic experiments in tissue construction, amyotrophic lateral sclerosis, transgenic mouse, morbid animal model, germline deterioration, breeding, National Natural Science Foundation of China

CLC Number:

R318

Cai Bin, Fan Dong-sheng. Germline degradation of a mouse model of familial amyotrophic lateral sclerosis when breeding[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(24): 4521-4528.

Figures/Tables 4

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