Expression of adenovirus-mediated nerve growth factor and myelin-associated glycoprotein double-gene in sciatic nerve injury

doi:10.3969/j.issn.2095-4344.2017.20.012

Abstract

Abstract:

BACKGROUND: Nerve growth factor (NGF) and myelin-associated glycoprotein (MAG) can promote the repair of peripheral nerve. However, increasing the expression of NGF and MAG in the injured peripheral nerve by transgene technology has not been reported.

OBJECTIVE: To investigate the expression of NGF and MAG after adenovirus (Ad) mediated transfection in the injured sciatic nerve in rats.

METHODS: Forty Sprague-Dawley rats were randomly divided into normal, model, Ad, NGF and NGF-MAG groups. Sciatic nerve injury models were established in the latter four groups by cutting and suturing the right sciatic nerve. Afterwards, the rats in each group were subject to the intramuscular injection of normal saline, normal saline, Ad (1×10⁸ PFU), Ad-NGF (1×10⁸ PFU), and Ad-NGF-MAG (1×10⁸ PFU), respectively, once every 2 days, for consecutive three times.

RESULTS AND CONCLUSION: The expression level of NGF in the rat sciatic nerve in the Ad and normal groups was significantly higher than that in the NGF and NGF-MAG groups (P < 0.05). Compared with the Ad, NGF and normal groups, the expression level of MAG in the NGF-MAG group was significantly increased (P < 0.05). To conclude, the Ad-mediated NGF and MAG after double gene transfection were expressed effectively in the injured sciatic nerve in rats.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Nerve Growth Factor, Myelin-Associated Glycoprotein, Transfection, Tissue Engineering

CLC Number:

R318

Chen Yu, Deng Zhong-liang, Chen Shi-mou, Weng Zheng, Huang Fang. Expression of adenovirus-mediated nerve growth factor and myelin-associated glycoprotein double-gene in sciatic nerve injury[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(20): 3183-3189.

Figures/Tables 4

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