Effect of electroacupuncture on nerve growth factor and its receptor expression in facial nerve nucleus after facial nerve injury in rabbits

doi:10.12307/2022.232

Abstract

Abstract: BACKGROUND: Preliminary studies have found that peripheral facial nerve injury can cause changes in the expression of nerve growth factor, but the mechanism of electroacupuncture in the expression of nerve growth factor is unclear.
OBJECTIVE: Based on a model of peripheral facial nerve injury in rabbits, to observe the effects of electroacupuncture on facial paralysis symptoms, morphology of facial nerve nuclei and expression of nerve growth factor and its receptor and to explore the mechanism of electroacupuncture in the treatment of peripheral facial nerve injury.
METHODS: Forty-two New Zealand white rabbits (n=66) were randomized into normal group (n=6), model group (n=30) and electroacupuncture group (n=30). A crushing injury model of superior buccal branch of the right facial nerve was established in the latter two groups. There was no deal with normal group. After successful modeling, a needle was imbedded at Hegu in the electroacpuncture group, followed by electric stimulations with dilatational wave at Jiache, Dicang, Sibai, Yangbai, Yifeng, and Quanliao. No treatment was done in the model group. At 28 days of electroacupuncture, morphological changes of facial nerve nuclei were observed using hematoxylin-eosin staining and the expression of nerve growth factor, TrkA and p75NTR in facial nerve nuclei was detected using immunohistochemical staining. The expression of nerve growth factor, TrkA and p75NTR mRNA was detected using fluorescent quantitative PCR. The study protocol was approved by the Animal Ethics Committee of Southwest Medical University, with an approval No. 20170120001.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining results showed that the morphology of neurons recovered faster and more completely in the electroacupuncture group than the model group after surgery. The results of immunohistochemical staining showed that the average absorbance value of nerve growth factor in the electroacupuncture group was higher than that in the model group at every time point (P < 0.05), and the average absorbance value of TrkA and p75NTR in the electroacupuncture group was higher than that in the model group on the 7th, 14th, and 28th days after surgery (P < 0.05). Fluorescent quantitative PCR results revealed that the relative expression of nerve growth factor and p75NTR mRNA in the electroacupuncture group was higher than that in the model group on the 14th and 28th days after surgery (P < 0.05). The TrkA mRNA expression in the electroacupuncure group was higher than that in the model group on the 7th, 14th, and 28th days after surgery (P < 0.05). To conclude, electroacupuncture can improve the expression of nerve growth factor, TrkA, and p75NTR in facial nerve nuclei, and may promote the binding between nerve growth factor with TrkA and p75NTR, which may be the mechanism by which electroacupuncture promotes the repair of facial nerve injury.

Key words: electroacupuncture, acupoint, peripheral facial paralysis, facial nerve nucleus, nerve growth factor, p75 nerve growth factor receptor, nerve growth factor tyrosine kinase receptor A

CLC Number:

Xiao Yang, Gong Liqiong, Fei Jing, Li Leiji. Effect of electroacupuncture on nerve growth factor and its receptor expression in facial nerve nucleus after facial nerve injury in rabbits[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(8): 1253-1259.

Figures/Tables 10

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