Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (18): 2802-2810.doi: 10.3969/j.issn.2095-4344.2017.18.003
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Received:
2017-02-11
Online:
2017-06-28
Published:
2017-07-07
About author:
Chen Xiao-min, Master, Associate professor, Shuqing Medical College, Zhengzhou 450064, Henan Province, China
CLC Number:
Chen Xiao-min, Ma Xiao, Shao Nan-qi, Dai Yong-xia, Gao Qing, Fu Jin-fang . Nano-hydroxyapatite/silk fibroin composite materials loaded with recombinant human bone morphogenetic protein 2 used for spinal fusion[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(18): 2802-2810.
2.2 实验动物一般情况及标本大体观察结果 术后所有实验动物一般情况良好,未出现腹泻、伤口感染、移植物排斥等明显不良反应。自体骨组1只动物出现功能障碍,均于1周内恢复。nHA/SF组和rhBMP-2/nHA/SF组各有1只切口出现清水样渗出物,经处理后自愈。所有实验动物无一只死亡。术后12周处死实验动物后重点观察各组标本融合部位,质地均较硬,其中自体骨组大量骨痂形成,可见骨连接,融合迹象明显。nHA/SF组可见骨痂,但骨性连接不坚固,未见融合迹象。rhBMP-2/nHA/SF组材料与周围肌肉组织结合比较紧密,部分骨痂形成,已有骨连接。 2.3 放射学检查结果 见图2和表1。自体骨组:椎体与髂骨植骨块分界处有大量骨小梁长入,密度高,X射线评估有10只符合融合标准。nHA/SF组:双侧L4/5椎体与纳米羟基磷灰石/丝素蛋白复合材料分界处有少量骨小梁长入,X射线评估有3只符合融合标准。rhBMP-2/nHA/SF组:骨小梁较多,X射线评估有9只符合融合标准。"
2.5 组织学观察及半定量分析结果 见图3。自体骨组:髂骨周边可见大量新骨形成,可见骨性连接。nHA/SF组:材料小部分降解,与周边交界处可见骨性连接,内部有新生编织骨,未见成熟骨组织。rhBMP-2/nHA/SF组:材料大部分降解,周边有大量新生毛细血管,并渗入组织内部,交界处未见纤维囊。经半定量分析后发现,自体骨组的新骨形成面积占(0.52±0.03)%,nHA/SF组的新骨形成面积占(0.41±0.02)%,rhBMP-2/nHA/SF组的新骨形成面积占(0.51±0.01)%。nHA/SF组比自体骨组低、rhBMP-2/ nHA/SF组比nHA/SF组高,差异均有显著性意义(P < 0.05)。"
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