Osteogenic ability of fascia- versus muscle-derived cells in rats

doi:10.3969/j.issn.2095-4344.2017.16.00

Abstract

Abstract:

BACKGROUND: Multipotent differentiation ability enables mesenchymal stem cells from autologous bone marrow to differentiate into osteoblasts and chondroblasts, thereby promoting the formation of bones and cartilage. However, the osteogenic ability differs from each other, and whose osteogenic ability is the best still needs to be studied further.
OBJECTIVE: To compare the osteogenic ability of fascia- and muscle-derived stem cells in rats.
METHODS: Fascia- and muscle-derived cells were isolated from 20 rats, followed by flow cytometry sorting, and were then cultured. FDC-LacZ cells were transfected with retro-BMP4 virus twice. Afterwards, the transfection efficiency of fascia-derived cells was detected through LacZ and alkaline phosphatase staining.
RESULTS AND CONCLUSION: Compared with fascia-derived cells, muscle-derived cells showed stronger chondrogenic ability and produced more calcium deposition. These findings indicate that the osteogenic ability of muscle-derived cells is superior to that of fascia-derived cells in rats.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Muscle Cells, Cell Differentiation, Tissue Engineering

CLC Number:

R318

Su Hai-bin, Li Guang-heng. Osteogenic ability of fascia- versus muscle-derived cells in rats[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(16): 2472-2477.

Figures/Tables 2

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