Effect of continuous versus intermittent force on the proliferation and differentiation of bone marrow stromal stem cells

doi:10.3969/j.issn.2095-4344.2017.13.001

Abstract

Abstract:

BACKGROUND: In the process of distraction osteogenisis, continuous and intermittent force can be used. However, which one is more beneficial for bone formation is still controversial.
OBJECTIVE: To investigate and compare the effect of continuous and intermittent force on the proliferation and differentiation of bone marrow stromal stem cells (BMSCs).
METHODS: Rabbit BMSCs were obtained using density gradient centrifugation method. The passage 3 BMSCs were seeded onto 6-well cell culture plates for mechanical investigation. Then, the cells were randomly divided into three groups. Group A was a control group without any treatments. Group B was the continuous force group with a centrifugal force (1 100 r/min, relative centrifugal force 20×g) applied 4 hours per day. Group C was the intermittent group with an centrifugal force (1 100 r/min, relative centrifugal force 100×g) applied twice in 4 hours and lasting 24 minutes once. After 3 days of culture under mechanical force, the proliferation of BMSCs and the expression of alkaline phosphatase and osteocalcin were detected and compared.
RESULTS AND CONCLUSION: The centrifugal force promoted the ostegenic differentiation and proliferation of BMSCs and the expression of alkaline phosphatase significantly increased compared with the control group (P < 0.05). Compared with the intermittent force, the continuous force showed better effect on osteogenic differentiation and proliferation of BMSCs as well as the activity of alkaline phosphatase (P < 0.05).

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Mechanotransduction, Cellular, Cell Proliferation, Alkaline Phosphatase, Osteocalcin, Tissue Engineering

CLC Number:

R394.2

Chen Jun-liang, He Yun, Xiao Jin-gang. Effect of continuous versus intermittent force on the proliferation and differentiation of bone marrow stromal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(13): 1969-1973.

Figures/Tables 4

References

[1] 陈宇轩,韩小宪,牛学刚,等.牵张成骨技术在治疗疑难牙颌面畸形中的应用[J].实用口腔医学杂志,2015,31(6):815-820.

[2] Flores RL, Tholpady SS, Sati S,et al.The surgical correction of Pierre Robin sequence: mandibular distraction osteogenesis versus tongue-lip adhesion.Plast Reconstr Surg. 2014;133(6):1433-1439.

[3] 张志愿,俞光岩.口腔颌面外科学[M].北京:人民卫生出版社,2012: 529-538.

[4] Xu H, Ke K, Zhang Z,et al.Effects of platelet-rich plasma and recombinant human bone morphogenetic protein-2 on suture distraction osteogenesis.J Craniofac Surg. 2013;24(2):645-650.

[5] Paschos NK, Brown WE, Eswaramoorthy R,et al.Advances in tissue engineering through stem cell-based co-culture.J Tissue Eng Regen Med. 2015;9(5):488-503.

[6] Nakamura T, Sekiya I, Muneta T,et al. Arthroscopic, histological and MRI analyses of cartilage repair after a minimally invasive method of transplantation of allogeneic synovial mesenchymal stromal cells into cartilage defects in pigs.Cytotherapy. 2012;14(3):327-338.

[7] 张鹏,江凌勇,房兵.机械刺激对体外骨髓基质干细胞骨向分化作用的研究进展[J].中国口腔颌面外科杂志,2012,10(1):81-85.

[8] Chen JL, Hunt P, McElvain M,et al.Osteoblast precursor cells are found in CD34+ cells from human bone marrow.Stem Cells. 1997;15(5):368-377.

[9] 段峰,关键,杨红岩,等.机械离心力对成骨细胞骨形态发生蛋白信号通路中Runx-2 mRNA的影响[J].中国组织工程研究与临床康复,2014,18(33):5305-5309.

[10] Manikandhan R, Lakshminarayana G, Sneha P,et al.Impact of mandibular distraction osteogenesis on the oropharyngeal airway in adult patients with obstructive sleep apnea secondary to retroglossal airway obstruction.J Maxillofac Oral Surg. 2014;13(2):92-98.

[11] Tee BC, Sun Z.Mandibular distraction osteogenesis assisted by cell-based tissue engineering: a systematic review.Orthod Craniofac Res. 2015;18 Suppl 1:39-49.

[12] Sun JJ, Zheng XH, Wang LY,et al.New bone formation enhanced by ADSCs overexpressing hRunx2 during mandibular distraction osteogenesis in osteoporotic rabbits.J Orthop Res. 2014;32(5):709-720.

[13] Li C, Ding J, Jiang L,et al.Potential of mesenchymal stem cells by adenovirus-mediated erythropoietin gene therapy approaches for bone defect.Cell Biochem Biophys. 2014; 70(2):1199-1204.

[14] Sun M, Tan W, Wang K,et al.Effects of allogenous periosteal-derived cells transfected with adenovirus-mediated BMP-2 on repairing defects of the mandible in rabbits.J Oral Maxillofac Surg. 2013;71(10):1789-1799.

[15] Deshpande SS, Gallagher KK, Donneys A,et al.Stem cell therapy remediates reconstruction of the craniofacial skeleton after radiation therapy.Stem Cells Dev. 2013;22(11): 1625-1632.

[16] Wang L, Zhao Y, Cheng X,et al.Effects of locally applied nerve growth factor to the inferior alveolar nerve histology in a rabbit model of mandibular distraction osteogenesis.Int J Oral Maxillofac Surg. 2009;38(1):64-69.

[17] Hariri F, Rahman ZA, Mahdah S, et al.A Novel Technique Using Customized Headgear for Fixation of Rigid External Distraction Device in an Infant With Crouzon Syndrome.J Craniofac Surg. 2015;26(8):e740-744.

[18] Dobbs TD, Wall SA, Richards PG,et al.A novel technique to secure the Rigid External Distraction (RED) frame in a thin skull allowing sutural mid-face distraction.J Craniomaxillofac Surg. 2014;42(7):1048-1051.

[19] Tehranchi A.Stability and effects of mandibular symphyseal distraction osteogenesis: a systematic review.British Journal of Medicine & Medical Research. 2015;7(8):688-698.

[20] Hu J, Li J, Wang D, Buckley MJ, et al. Differences in mandibular distraction osteogenesis after corticotomy and osteotomy. Int J Oral Maxillofac Surg. 2002;31(2):185-189..

[21] Wiltfang J, Kessler P, Merten HA, et al. Continuous and intermittent bone distraction using a microhydraulic cylinder: an experimental study in minipigs. Br J Oral Maxillofac Surg. 2001;39(1):2-7.

[22] Aloise AC, Pelegrine AA, Zimmermann A, et al. Repair of critical-size bone defects using bone marrow stem cells or autogenous bone with or without collagen membrane: a histomorphometric study in rabbit calvaria. Int J Oral Maxillofac Implants. 2015;30(1):208-215.

[23] Wang Y, Bi X, Zhou H, et al. Repair of orbital bone defects in canines using grafts of enriched autologous bone marrow stromal cells. J Transl Med. 2014;12:123.

[24] Gulseren G, Yasa IC, Ustahuseyin O, et al. Alkaline Phosphatase-Mimicking Peptide Nanofibers for Osteogenic Differentiation. Biomacromolecules. 2015;16(7):2198-2208.

[25] Wei J, Karsenty G. An overview of the metabolic functions of osteocalcin. Curr Osteoporos Rep. 2015;13(3):180-185.

[26] 赵永亮.不同时期离心力对小鼠骨髓间充质干细胞向软骨分化的实验研究[D].太原:山西医科大学, 2016.

[27] Lee DH, Park JC, Suh H. Effect of centrifugal force on cellular activity of osteoblastic MC3T3-E1 cells in vitro. Yonsei Med J. 2001;42(4):405-410.

[28] Inoue H, Nakamura O, Duan Y, et al. Effect of centrifugal force on growth of mouse osteoblastic MC3T3-E1 cells in vitro. J Dent Res. 1993;72(9):1351-1355.

[29] Fitzgerald J, Hughes-Fulford M. Mechanically induced c-fos expression is mediated by cAMP in MC3T3-E1 osteoblasts. FASEB J. 1999;13(3):553-557.