Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (13): 1969-1973.doi: 10.3969/j.issn.2095-4344.2017.13.001

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Effect of continuous versus intermittent force on the proliferation and differentiation of bone marrow stromal stem cells

Chen Jun-liang1, 2, He Yun1, 2, Xiao Jin-gang1, 2   

  1. 1Department of Oral and Maxillofacial Surgery, the Hospital of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China; 2Orofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Revised:2017-03-11 Online:2017-05-08 Published:2017-06-09
  • Contact: He Yun, Master, Associate professor, Department of Oral and Maxillofacial Surgery, the Hospital of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Orofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • About author:Chen Jun-liang, Master, Attending physician, Department of Oral and Maxillofacial Surgery, the Hospital of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Orofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81371125; the Project of the Hospital of Stomatology, Southwest Medical University, No. 20130412

Abstract:

BACKGROUND: In the process of distraction osteogenisis, continuous and intermittent force can be used. However, which one is more beneficial for bone formation is still controversial.
OBJECTIVE: To investigate and compare the effect of continuous and intermittent force on the proliferation and differentiation of bone marrow stromal stem cells (BMSCs).
METHODS: Rabbit BMSCs were obtained using density gradient centrifugation method. The passage 3 BMSCs were seeded onto 6-well cell culture plates for mechanical investigation. Then, the cells were randomly divided into three groups. Group A was a control group without any treatments. Group B was the continuous force group with a centrifugal force (1 100 r/min, relative centrifugal force 20×g) applied 4 hours per day. Group C was the intermittent group with an centrifugal force (1 100 r/min, relative centrifugal force 100×g) applied twice in 4 hours and lasting 24 minutes once. After 3 days of culture under mechanical force, the proliferation of BMSCs and the expression of alkaline phosphatase and osteocalcin were detected and compared.
RESULTS AND CONCLUSION: The centrifugal force promoted the ostegenic differentiation and proliferation of BMSCs and the expression of alkaline phosphatase significantly increased compared with the control group (P < 0.05). Compared with the intermittent force, the continuous force showed better effect on osteogenic differentiation and proliferation of BMSCs as well as the activity of alkaline phosphatase (P < 0.05).

 

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Mechanotransduction, Cellular, Cell Proliferation, Alkaline Phosphatase, Osteocalcin, Tissue Engineering

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