Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (52): 7836-7843.doi: 10.3969/j.issn.2095-4344.2016.52.012
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Received:
2016-09-25
Online:
2016-12-16
Published:
2016-12-16
Contact:
Guo Kai-jin, Professor, Department of Spine Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221004, Jiangsu Province, China
About author:
Liu Guo-dong, Studying for master’s degree, Graduate School of Xuzhou Medical University, Xuzhou 221004, Jiangsu Province, China
Supported by:
the Science Research Foundation of the Health Department, No. LW201004; the Science and Technology Project of Xuzhou, No. XM13B080
CLC Number:
Liu Guo-dong, Xin Bing, Huang Dong, Zheng Bai, Sun Bai-han, Guo Kai-jin.
2.1 实验动物数量分析 48只昆明小鼠均进入结果分析。 2.2 苏木伊红染色结果 正常小鼠背部皮肤组织真皮层中可见毛囊和皮脂腺细胞、毛囊陷窝等处未见炎性细胞浸润,皮下疏松结缔组织亦未见炎性细胞浸润,见图4A。与正常组比较,钛颗粒组小鼠背部皮肤真皮层毛囊陷窝处及皮下疏松结缔组织可见大量炎性细胞浸润及空泡坏死组织,见图4B。与正常组比较,DMSO组小鼠背部皮肤真皮层毛囊陷窝处及皮下疏松结缔组织也可见大量炎性细胞浸润及空泡坏死组织,所见基本和钛颗粒组相似,见图4C。实验组小鼠背部皮肤真皮层毛囊陷窝处及皮下疏松结缔组织炎性细胞的浸润较钛颗粒组和DMSO组明显减少,空泡坏死组织也有所减少,见图4D。"
2.3.2 抑制性卡巴蛋白、NF-κB 在钛颗粒诱导小鼠背部气囊炎症模型中,NF-κB是激活诱导型一氧化氮合酶、环氧化酶2和细胞因子表达的重要调节,因此用免疫印迹法检测NF-κB、抑制性卡巴蛋白的表达变化。钛颗粒可以诱导NF-κB的大量表达,且在溶媒剂DMSO组其表达量不变,而在注入亚油酸乙酯14 d后,可观察到NF-κB的表达明显减少。在正常组中,抑制性卡巴蛋白大量表达;经钛颗粒诱导后,抑制性卡巴蛋白表达明显下降,DMSO组无明显变化;然而,与钛颗粒组比较,实验组抑制性卡巴蛋白表达显著升高,见图6。结果表明,经钛颗粒的刺激会加速抑制性卡巴蛋白磷酸化降解及与NF-κB的解离,而亚油酸乙酯则可抑制抑制性卡巴蛋白的磷酸化降解,使ⅠκB-α表达增高,进一步抑制NF-κB的激活。"
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