Biological characteristics of bone marrow mesenchymal stem cells and application in skin defect repair

doi:10.3969/j.issn.2095-4344.2016.50.012

Abstract

Abstract:

BACKGROUND: An appropriate biomaterial can be directly combined with autologous or allogeneic skin cells to construct tissue-engineered skin, which can accelerate skin repair after transplantation onto the skin wounds. It is a good idea to solve the deficiency in skin sources.
OBJECTIVE: To study the biological characteristics of the complex with bone marrow mesenchymal stem cells and to investigate the application of this complex in skin defect repair.
METHODS: Twenty-eight Sprague-Dawley rats, SPF grade, were randomized into two groups (n=14 per group). By adjustment of temperature, time, pressure and area of NC perm instrument, scald models were made in rats. Rats in the treatment group were given the repair using composite bone marrow mesenchymal stem cells, while those in the control group were given vaseline gauze repair. Repair effects were compared between two groups.
RESULTS AND CONCLUSION: (1) After 72 hours of culture, the composite bone marrow mesenchymal stem cells small with round shape distributed dispersedly. After 5 days of culture, the cells began to stretch and the cell morphology became unstable. After three passages, the cell morphology became stable. The results of antigen identification showed the expression of CD44 and CD29 but the low expression of CD45 and CD34 in bone marrow mesenchymal stem cells. (2) Twenty-eight days after repair, there was no obvious scar on the wound surface of the treatment group, but a little shrinkage and obvious scar still existed in the control group. Moreover, in the control group, the epidermal layer of the skin was relatively thick, and the connection between the basal layer and the dermis was unsatisfactory. In the treatment group, obvious epidermal cell stratification, neat arrangement, and tight connection between the epidermis and dermis were observed. In summary, bone marrow mesenchymal stem cells are a special class of cells that have pluripotent ability and are more readily available. These cells are the preferred target cells for skin defect repair to promote early healing of the skin and improve blood circulation defect site, which are confirmed to have high clinical value.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Skin, Artificial, Biocompatible Materials, Tissue Engineering

CLC Number:

R394.2

Du Peng, Wang Xiao-chuan, Guan Zhen. Biological characteristics of bone marrow mesenchymal stem cells and application in skin defect repair[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(50): 7530-7535.

Figures/Tables 1

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