Effects of Bergapten on the aging of human umbilical vein endothelial cells induced by hydrogen peroxide

doi:10.3969/j.issn.2095-4344.2016.46.006

Abstract

Abstract:

BACKGROUND: The effective treatment of traditional Chinese medicine can alleviate the aging of endothelial cells. Traditional Chinese medicine is difficult to extract, and in contrast, Bergapten is cheap and easily purified, but its antisenescence is little reported.
OBJECTIVE: To explore the effect of Bergapten on H2O2-induced senescence of human umbilical vein endothelial cells.
METHODS: Human umbilical vein cell aging model was induced by H2O2, and pretreated with 0.1, 1 and 10 μmol/L Bergapten, respectively. Expressions of pRb, pAmpk, pS6, LC3-II, and Beclin-1 protein in each group were detected by western blot assay, and the number of aging cells and pS6-positive cells was determined by β-galactosidase staining and immunofluorescence, respectively.
ESULTS AND CONCLUSION: After Bergapten pretreatment, pS6 and pRb protein expressions were significantly downregulated, and the number of cells positive for β-galactosidase and pS6 was significantly decreased, while protein expressions of pAmpk, Beclin-1and LC3-II were significantly increased (P < 0.05). These findings suggest that Bergapten is able to delay the H2O2-induced aging of human umbilical vein endothelial cells

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Tissue Engineering, Cell Aging, Endothelial Cells

CLC Number:

R318

Ding Yan, Fu You, Shan Lan-lan, Nai Wen-qing, Wu Hong-yuan, Tang Liang, Chen Shun-zhi, Dai Meng. Effects of Bergapten on the aging of human umbilical vein endothelial cells induced by hydrogen peroxide[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(46): 6885-6892.

Figures/Tables 3

References

[1] Limbourg FP, Takeshita K, Radtke F, et al. Essential role of endothelial Notch1 in angiogenesis. Circulation. 2005;111(14):1826-1832.
[2] Sengupta A, Chakraborty S, Paik J, et al. FoxO1 is required in endothelial but not myocardial cell lineages during cardiovascular development. Dev Dyn. 2012; 241(4):803-813.
[3] Potente M, Ghaeni L, Baldessari D, et al. SIRT1 controls endothelial angiogenic functions during vascular growth. Genes Dev. 2007;21(20):2644-2658.
[4] Tan W, Palmby TR, Gavard J, et al. An essential role for Rac1 in endothelial cell function and vascular development. FASEB J. 2008;22(6):1829-1838.
[5] Gepner AD, Korcarz CE, Colangelo LA, et al. Longitudinal effects of a decade of aging on carotid artery stiffness: the multiethnic study of atherosclerosis. Stroke. 2014;45(1):48-53.
[6] Piotrowska A, Bartnik E. The role of reactive oxygen species and mitochondria in aging. Postepy Biochem. 2014;60(2):240-247.
[7] 王宇霆,周海燕,吕小翠.白藜芦醇对过氧化氢氧化应激损伤人脐静脉血管内皮细胞的保护作用[J].中华医学杂志, 2013,93(15):1174-1177.
[8] Tian F, Yu B, Hu J. mTOR mediates the cross-talk of macrophage polarization and autophagy in atherosclerosis. Int J Cardiol. 2014;177(1):144-145.
[9] Laplante M, Sabatini DM. Regulation of mTORC1 and its impact on gene expression at a glance. J Cell Sci. 2013;126(Pt 8):1713-1719.
[10] 秦毅,王彦凤,王志钢.TSC1/TSC2二聚体及其在细胞生长调控中的作用[J].生物技术通报,2013,29(9):38-42.
[11] 陈银涛,于秉治,武迪迪.PI3K/Akt/mTOR信号通路及临床相关肿瘤抑制剂[J].中国生物化学与分子生物学报,2014, 30(10):949-956.
[12] Maiuri MC, Zalckvar E, Kimchi A, et al. Self-eating and self-killing: crosstalk between autophagy and apoptosis. Nat Rev Mol Cell Biol. 2007;8(9):741-752.
[13] Gwinn DM, Shackelford DB, Egan DF, et al. AMPK phosphorylation of raptor mediates a metabolic checkpoint. Mol Cell. 2008;30(2):214-226.
[14] Sarbassov DD, Ali SM, Sabatini DM. Growing roles for the mTOR pathway. Curr Opin Cell Biol. 2005;17(6): 596-603.
[15] Xu Y, Li N, Xiang R, et al. Emerging roles of the p38 MAPK and PI3K/AKT/mTOR pathways in oncogene-induced senescence. Trends Biochem Sci. 2014;39(6):268-276.
[16] Zoncu R, Efeyan A, Sabatini DM. mTOR: from growth signal integration to cancer, diabetes and ageing. Nat Rev Mol Cell Biol. 2011;12(1):21-35.
[17] Tan CC, Yu JT, Tan MS, et al. Autophagy in aging and neurodegenerative diseases: implications for pathogenesis and therapy. Neurobiol Aging. 2014; 35(5):941-957.
[18] 张创杰,张连峰,周琳.自噬相关蛋白Beclin1、LC3和P62在进展期胰腺癌中的表达及临床意义[J].世界华人消化杂志,2015,23(2):318-323.
[19] Datan E, Shirazian A, Benjamin S, et al. mTOR/p70S6K signaling distinguishes routine, maintenance-level autophagy from autophagic cell death during influenza A infection. Virology. 2014; 452-453:175-190.
[20] Tall AR, Yvan-Charvet L. Cholesterol, inflammation and innate immunity. Nat Rev Immunol. 2015;15(2): 104-116.
[21] 余绍蕾,白莉,蔡宇.补骨脂素对肿瘤血管内皮细胞抑制作用的实验研究[J].数理医药学杂志,2013,26(4):458-459.
[22] 王志国,何德,金洪,等.无花果抗癌作用的研究进展[J].现代生物医学进展,2010,10(11):2183-2186.
[23] 崔秋兵,张艺,兰莎.白芷镇痛作用物质基础研究[J].中国实验方剂学杂志,2010,16(12):102-104.
[24] Qian GS, Wang Q, Leung KS, et al. Quality assessment of Rhizoma et Radix Notopterygii by HPTLC and HPLC fingerprinting and HPLC quantitative analysis. J Pharm Biomed Anal. 2007;44(3):812-817.
[25] 林碧华,万树伟,刘付梅,等.佛手柑内酯对鼻咽癌细胞周期的影响[J].中国药学杂志,2014,49(10):837-842.
[26] Chen F, Chen B, Xiao F, et al. Autophagy protects against senescence and apoptosis via the ras-mitochondria in high-glucose-induced endothelial cells. Cell Physiol Biochem. 2014;33(4):1058-1074.
[27] 蔡维霞,梁亮,计鹏,等.人脐静脉血管内皮细胞体外培养的方法研究[J].中国修复重建外科杂志,2011,25(2): 139-143.
[28] 刘长乐,郑心田,李广平,等.体外人脐静脉内皮细胞的培养鉴定及抗原表达分析[J].山东医药,2011,50(36): 25-26.
[29] Salminen A, Kaarniranta K, Kauppinen A. Beclin 1 interactome controls the crosstalk between apoptosis, autophagy and inflammasome activation: impact on the aging process. Ageing Res Rev. 2013;12(2): 520-534.
[30] 刘辉,陈桐帅,李娜,等.Sirt3对人脐静脉内皮细胞衰老的影响[J].山东大学学报(医学版),2015,53(5):41-45.
[31] Hu J, Wang X, Wei SM, et al. Activin A stimulates the proliferation and differentiation of cardiac fibroblasts via the ERK1/2 and p38-MAPK pathways. Eur J Pharmacol. 2016;789:319-327.
[32] 辛培成,杨圣,芦健民,等.CCK-8法检测兔筋膜成纤维细胞增殖活性之最佳测试条件研究[J].中外医学研究, 2011, 9(12):6-8.
[33] Gilda JE, Gomes AV. Western blotting using in-gel protein labeling as a normalization control: stain-free technology. Methods Mol Biol. 2015;1295:381-391.
[34] Xiao X, Chen ZK, Ding YQ, et al. Senescence-associated beta galactosidase expression in rat schwann cells after chronic denervation. Sichuan Da Xue Xue Bao Yi Xue Ban. 2016;47(3):351-355.
[35] Holle L, Song W, Hicks L, et al. In vitro targeted killing of human endothelial cells by co-incubation of human serum and NGR peptide conjugated human albumin protein bearing alpha (1-3) galactose epitopes. Oncol Rep. 2004;11(3):613-616.
[36] Verma M, Murkonda BS, Asakura Y, et al. Skeletal muscle tissue clearing for lacz and fluorescent reporters, and immunofluorescence staining. Methods Mol Biol. 2016;1460:129-140.
[37] Vogler TO, Gadek KE, Cadwallader AB, et al. Isolation, culture, functional assays, and immunofluorescence of myofiber-associated satellite cells. Methods Mol Biol. 2016;1460:141-162.
[38] Ai D, Jiang H, Westerterp M, et al. Disruption of mammalian target of rapamycin complex 1 in macrophages decreases chemokine gene expression and atherosclerosis. Circ Res. 2014;114(10): 1576-1584.
[39] Solon-Biet SM, Mcmahon AC, Ballard JW, et al. The ratio of macronutrients, not caloric intake, dictates cardiometabolic health, aging, and longevity in ad libitum-fed mice. Cell Metab. 2014;19(3):418-430.
[40] Peart JN, PepeS, Reichelt ME, et al. Dysfunctional survival-signaling and stress-intolerance in aged murine and human myocardium. Exp Gerontol. 2014; 50:72-81.
[41] Sawada N, Jiang A, Takizawa F, et al. Endothelial PGC-1alpha mediates vascular dysfunction in diabetes. Cell Metab. 2014;19(2):246-258.
[42] Mowla SN, Lam EW, Jat PS. Cellular senescence and aging: the role of B-MYB. Aging Cell. 2014;13(5): 773-779.
[43] 昌艳艳,吕晓东,王乐亭,等.中药对过氧化氢诱导人脐静脉内皮细胞株损伤的作用影响[J].中华中医药学刊,2008, 26(3):549-550.
[44] 陈永兰.丹参黄芪注射液合用治疗冠心病心绞痛的疗效观察[J].北方药学,2012,8(11):4-5.
[45] 钟佩茹,高秀梅,陈彤,等.补骨脂素对人脐静脉内皮细胞活力及TNF-α诱导组织因子产生的影响[J].辽宁中医杂志, 2012,42(11):2247-2249.
[46] Lamming DW, Mihaylova MM, Katajisto P, et al. Depletion of Rictor, an essential protein component of mTORC2, decreases male lifespan. Aging Cell. 2014; 13(5):911-917.
[47] Shafique E, Choy W C, Liu Y, et al. Oxidative stress improves coronary endothelial function through activation of the pro-survival kinase AMPK. Aging (Albany NY). 2013;5(7):515-530.
[48] Laplante M, Sabatini DM. mTOR signaling at a glance. J Cell Sci. 2009;122(Pt 20):3589-3594.
[49] 翟纯刚,季晓平,王和峰,等.抑制PI3K/Akt/mTOR信号通路对兔原代巨噬细胞自体吞噬的影响及机制[J].中国动脉硬化杂志,2014,22(1):7-12.
[50] 陈家劲,王娟娟,张鹏,等.重楼活性单体PP-26抑制结肠癌SW620细胞增殖并诱导细胞凋亡[J].暨南大学学报(自然科学与医学版),2015,55(2):124-130.
[51] De Amicis F, Aquila S, Morelli C, et al. Bergapten drives autophagy through the up-regulation of PTEN expression in breast cancer cells. Mol Cancer. 2015; 14:130.
[52] Bauri AK, Foro S, Nhu Do QN. Crystal structure of bergapten: a photomutagenic and photobiologically active furan-ocoumarin. Acta Crystallogr E Crystallogr Commun. 2016;72(Pt 8):1194-1196.
[53] Tavolaro P, Martino G, Andò S, et al. Zeolite scaffolds for cultures of human breast cancer cells. Part II: Effect of pure and hybrid zeolite membranes on neoplastic and metastatic activity control. Mater Sci Eng C Mater Biol Appl. 2016;68:474-481.
[54] Zhao AH, Zhang YB, Yang XW. Simultaneous determination and pharmacokinetics of sixteen Angelicae dahurica coumarins in vivo by LC-ESI-MS/MS following oral delivery in rats. Phytomedicine. 2016;23(10):1029-1036.
[55] Lee SG, Kim K, Vance TM, et al. Development of a comprehensive analytical method for furanocoumarins in grapefruit and their metabolites in plasma and urine using UPLC-MS/MS: a preliminary study. Int J Food Sci Nutr. 2016:1-7.
[56] Zhou P, Xie CQ, Chen JW, et al. Effects of Browning Inhibitors on Suspension Cells Growth and Secondary Metabolites Production in Changium smyrnioides. Zhong Yao Cai. 2015;38(11):2250-2254.
[57] Zhao Y, Wang N, Zeng Z, et al. Cloning, Functional Characterization, and Catalytic Mechanism of a Bergaptol O-Methyltransferase from Peucedanum praeruptorum Dunn. Front Plant Sci. 2016;7:722.