A chemosynthetic medical adhesive containing alpha-N butyl cyanoacrylate for liver hemostasis

doi:10.3969/j.issn.2095-4344.2016.47.017

Abstract

Abstract:

BACKGROUND: There are chemosynthetic and biological medical adhesives, and the former mainly composed by cyanoacrylate, holds poor hydrophilicity and is easy to break off, thereby achieving unsatisfactory hemostatic effect.
OBJECTIVE: To evaluate the hemostatic and adhesion effect of the modified α-N butyl cyanoacrylate (NBCA) by the hemostatic test in white rabbit livers.
METHODS: Material imprvement: cyanoacrylate monomer was prepared and its components and contents were analyzed by infrared spectrum and gas chromatograph. Sodium lauroyl sarcosinate was used as surfactant to improve the cohesive force between NBCA and tissues and increase the hydrophility. Hemostatic test in the liver incision: the liver incision of rabbits in three groups were sewn with absorbable suture and then covered with gelatin sponge (control group), cyanoacrylate and NBCA medical adhesives, respectively. Blood loss and bleeding time were recorded within 20 minutes. Hemostatic test in the liver section: the liver sections in two groups were smeared with surfactant plus NBCA and cyanoacrylate, respectively. The gross appearance of wound and its adherence to the omentum majus, as well as the histological changes by hematoxylin-eosin staining were observed at 1, 10 and 20 days postoperatively.
RSULTS AND CONCLUSIPON: The adhesiveness of NBCA medical adhesive was excellent. Infrared spectrum and gas chromatograph found that the main component was NBCA up to 84.56%, with 8.2% reactants and 3.3% byproducts. The modified NBCA medical adhesive significantly decreased the blood loss and bleeding time compared with the control group (P < 0.01), and did not significantly differ from the cyanoacrylate (P > 0.05). In addition, the modified NBCA adhered more firmly to the wound, and was absorbed completely at 20 days postoperatively, showing no adverse reaction. These results suggest that NBCA is better and more stable than gelatin sponge, and exerts overt hemostatic effect causing no adverse reaction.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Biocompatible Materials, Hemostasis, Gelatin Sponge, Absorbable, Tissue Engineering

CLC Number:

R318

Zhang Fei, Zhao Jun, Meng Xing-kai, Li Jian-feng. A chemosynthetic medical adhesive containing alpha-N butyl cyanoacrylate for liver hemostasis[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(47): 7112-7118.

Figures/Tables 6

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