Comparative analysis of the proliferation and differentiation of neural stem cells in the hippocampal dentate gyrus of rats with different ages

doi:10.3969/j.issn.2095-4344.2016.36.016

Abstract

Abstract:

BACKGROUND: Cerebral hemorrhage can activate the proliferation and differentiation of neural stem cells in the dentate gyrus of the hippocampus. Through continuous differentiation and proliferation, endogenous neural stem cells can gradually replace aging and damaged neurons, thus protecting the brain structure.
OBJECTIVE: To compare the difference of the proliferation and differentiation of neural stem cells in the dentate gyrus of the hippocampus of rats with different ages.
METHODS: Ninety-six adult rats and 96 aged rats were randomly divided into normal group (n=18 per group), sham operation group (n=12 per group) and cerebral hemorrhage group (model group, n=66 per group), respectively. Cerebral hemorrhage models were made in the two model groups in which, the rats were subjected to cerebral hemorrhage for 6, 24, 48, 72 hours and 7 days, respectively. Then, brain tissues were collected to measure brain water content. BrdU/NeuN and BrdU/GFAP double staining were performed at 3, 7, 14, 21, 28 days after surgery to calculate the number of positive cells.
RESULTS AND CONCLUSION: For both adult and aged rats, the brain water content was significantly higher than that in the normal group and sham operation group (P < 0.05), while in the normal and sham operation groups, the brain water content was significantly lower in the aged rats than the adult rats (P < 0.05). The number of bilateral BrdU-positive cells in the adult and aged model groups was significantly higher than that in the corresponding normal and sham operation groups (P < 0.05), and moreover, the positive cell number at the hemorrhage side was significantly higher than that at the opposite side (P < 0.05). In addition, the number of BrdU-positive cells at the hemorrhage side in the adult rats was significantly higher than that in the aged rats at different time after cerebral hemorrhage (P < 0.05). Results from immunohistochemical double staining showed that the BrdU/NeuN and BrdU/GFAP expression in the hippocampal dentate gyrus of adult rats with cerebral hemorrhage was significantly higher than that of normal adult rats. All these experimental results show that there are a few neural stem cells proliferating in the hippocampal dentate gyrus of normal rats, and the proliferation ability is stronger in the adult rats than the aged rats. Cerebral hemorrhage can significantly strengthen the proliferation of neural stem cells in the dentate gyrus in the adult rats compared with the aged rats.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Cerebral Hemorrhage, Stem Cells, Neural Stem Cells, Hippocampus, Tissue Engineering

CLC Number:

R394.2

Xie Qiang, Wang Fei, Zhou Guo-ping, Zhang Hui, Ma Jin-xian . Comparative analysis of the proliferation and differentiation of neural stem cells in the hippocampal dentate gyrus of rats with different ages[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(36): 5426-5431.

Figures/Tables 1

2.1 实验流程与动物数量分析入组的动物均进入最后的结果分析，未出现死亡和脱落现象。 2.2 各组大鼠脑含水量检测结果对成年组和老年组大鼠的脑含水量进行检测，显示两组脑含水量均呈现出不断增加的情况，并于出血后72 h达到最大值，之后逐渐下降(见图1)。成年与老年大鼠脑出血组不同时间的脑含水量均显著高于正常组和假手术组(均P < 0.05)，正常组和假手术组经比较差异无显著性意义(P > 0.05)。老年组正常组和假手术组的脑含水量均显著低于成年组(均P < 0.05)。脑出血不同时间点老年组和成年组经比较差异无显著性意义(均P > 0.05)。见表1。 2.3 成年大鼠脑出血后海马齿状回BrdU阳性细胞数统计结果成年大鼠正常组和假手术组的海马齿状回BrdU 阳性细胞数经比较差异无显著性意义(均P > 0.05)。正常组和假手术组经比较差异无显著性意义(P > 0.05)。脑出血组不同时间的两侧BrdU 阳性细胞数均显著高于正常组和假手术组(均P < 0.05)，且出血组不同时间的出血侧BrdU 阳性细胞数均显著高于对侧(均P < 0.05)，见表2。 2.4 老年大鼠脑出血后海马齿状回BrdU阳性细胞数统计结果老年大鼠正常组和假手术组的海马齿状回BrdU 阳性细胞数经比较差异无显著性意义(均P > 0.05)。正常组和假手术组经比较差异无显著性意义(P > 0.05)。脑出血组不同时间的两侧BrdU 阳性细胞数均显著高于正常组和假手术组(均P < 0.05)，且出血组不同时间的出血侧BrdU 阳性细胞数均显著高于对侧(均P < 0.05)，见表3。 2.5 成年、老年大鼠脑出血后出血侧海马齿状回BrdU阳性细胞数统计结果成年大鼠脑出血后出血侧海马齿状回BrdU 阳性细胞数正常组、假手术组、脑出血组均显著高于相应的老年组大鼠(均P < 0.05)，见表4。 2.6 大鼠脑出血后海马齿状回神经干细胞免疫组织化学双标结果 ①成年大鼠脑出血后海马齿状回神经干细胞免疫疫组织化学双标结果显著高于正常组(P < 0.05)。②老年大鼠脑出血后海马齿状回神经干细胞免疫组织化学双标结果显著高于正常组(P < 0.05)。③成年大鼠海马齿状回神经干细胞免疫组织化学双标BrdU/NeuN结果显著高于老年大鼠，BrdU/GFAP 结果显著低于老年大鼠(均P < 0.05)。见表5。"

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