Tumor-killing effects of gastric cancer stem cells as antigens to stimulate dendritic cells combined with cytokine-induced killer cells

doi:10.3969/j.issn.2095-4344.2016.36.004

Abstract

Abstract:

BACKGROUND: As the existence of tumor stem cells, it is difficult to completely eliminate tumors in clinic.
OBJECTIVE: To explore the tumor-killing effect of gastric cancer stem cells as antigen to stimulate dendritic cells combined with cytokine-induced killer cells.
METHODS: Side population cells from human gastric cancer cell lines were isolated, and tumor antigen was prepared by freeze thawing method. After coculture with dendritic cells, dendritic cells combined with cytokine-induced killer cells, gastric cancer cell antigen, and gastric cancer stem cell antigen, killing rates of gastric cancer cells were detected using MTT assay. Expression rate of CD83, a mature dendritic cell surface marker, was also detected.
RESULTS AND CONCLUSION: The CD83 expression level and killing rate of gastric cancer cells were both significantly lower in the gastric cancer stem cell antigen group than the other groups (both P < 0.05). These results indicate that gastric cancer stem cells as antigen to stimulate dendritic cells combined with cytokine-induced killer cells can promote the proliferation of gastric cancer cells and elevate the ability to killing gastric cancer cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stomach Neoplasms, Neoplastic Stem Cells, Dendritic Cells, Tissue Engineering

CLC Number:

R394.2

Wen Li-hong, Hu Wen-jie, Ye Heng-xi, Xiang Wei-dong. Tumor-killing effects of gastric cancer stem cells as antigens to stimulate dendritic cells combined with cytokine-induced killer cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(36): 5345-5350.

Figures/Tables 1

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