Adenovirus-mediated brain-derived neurotrophic factor and endogenous neural stem cell differentiation in a rat model of cerebral hemorrhage

doi:10.3969/j.issn.2095-4344.2016.27.011

Abstract

Abstract:

BACKGROUND: Previous studies showed that neurotrophic factor has a variety of functions, which can effectively maintain the survival of neurons after injury.
OBJECTIVE: To observe the effect of adenovirus-mediated brain-derived neurotrophic factor on the differentiation of endogenous neural stem cells after intracerebral hemorrhage in rats.
METHODS: A total of 90 Sprague-Dawley rat models of cerebral hemorrhage were made. At 12 hours after cerebral hemorrhage, 5-bromodeoxyuridine (BrdU) was intraperitoneally injected, twice a day, for 10 consecutive days. After model establishment, rats were randomly divided into three groups, 30 rats in each group, and were respectively subjected to brain stereotaxic injection of adenovirus vector, adenovirus-mediated brain-derived neurotrophic factor and physiological saline. At 1 day, 3 days, 1 week, 2 weeks, 3 weeks, and 4 weeks, neurological deficit score was evaluated. Absorbance value of growth associated protein around the area of hematoma after intracerebral hemorrhage was measured. At 4 weeks after injection, double immunostaining was used to detect the expression of BrdU/NeuN and BrdU/glial fibrillary acidic protein (GFAP).
RESULTS AND CONCLUSION: (1) With the passage of time, nerve function defect score decreased in the three groups. At 1-4 weeks after injection, nerve function deficit scores were lower in the adenovirus-mediated brain-derived neurotrophic factor group than that in the adenovirus vector group and saline group (P < 0.05). (2) With the passage of time, the average absorbance of three groups in the peri-hematoma region first increased and then decreased. The absorbance value was higher in the adenovirus-mediated brain-derived neurotrophic factor group than in the adenovirus vector group and saline group at 3 days-4 weeks (P < 0.05). (3) BrdU/NeuN and BrdU/GFAP rates were significantly higher in the adenovirus-mediated brain-derived neurotrophic factor group than that of adenovirus vector group and saline group (P < 0.05). (4) The results show that the brain-derived neurotrophic factor mediated by adenovirus, and intervention on cerebral hemorrhage in rats can effectively promote the differentiation of endogenous neural stem cells, and promote the recovery of neural function in animal.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Cerebral Hemorrhage, Brain-Derived Neurotrophic Factor, Neural Stem Cells, Tissue Engineering

CLC Number:

R318

Xie Qiang, Wang Fei, Zhou Guo-ping, Zhang Hui, Ma Jin-xian. Adenovirus-mediated brain-derived neurotrophic factor and endogenous neural stem cell differentiation in a rat model of cerebral hemorrhage[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(27): 4029-4035.

Figures/Tables 3

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