hIL-24 gene influences the biological characteristics of the keloid by regulating transforming growth factor-beta/Smad pathway

doi:10.3969/j.issn.2095-4344.2016.33.009

Abstract

Abstract:

BACKGROUND: hIL-24, a tumor suppressor gene, can stimulate immune responses, inhibit the growth of tumor cells, and the formation of tumor vessels, and induce cell apoptosis.
OBJECTIVE: To explore the effects of hIL-24 gene on the proliferation and apoptosis of fibroblasts in the keloid and the underlying mechanisms.
METHODS: All the keloid specimens collected from 13 patients were used for fibroblast culture and indentification. Fibroblast of the keloid was transfected with or without hlL-24 lentivirus. Subsequently, mRNA expressions of transforming growth factor-β, Smad3, proliferating cell nuclear antigen, matrix metalloproteinase-2, -9, and metallopeptidase inhibitor 1 were determined.
RESULTS AND CONCLUSION: Immunofluorescent staining and flow cytometry showed that vimentin antibody was expressed positively in cytoplasma of fibroblast cultures, and the purity was more than 97.8%. Western blot assay showed that hIL-24 expression was significantly increased in the transfected fibroblasts. Quantitative PCR showed that the overexpression rate of hIL-24 in fibroblasts was 81.7% and mRNA expressions of transforming growth factor-β, Smad3, proliferating cell nuclear antigen, matrix metalloproteinase-2, and -9 were significantly decreased, while metallopeptidase inhibitor 1 mRNA expression was significantly increased in hIL-24 transfection group compared with control group (P < 0.05). These findings suggest that hIL-24 gene inhibits the expressions of proliferating cell nuclear antigen, matrix metalloproteinase-2, and -9 in fibroblasts, and the underlying mechanism may involves TGF-β/Smad3 pathway.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Keloid, Matrix Metalloproteinases, Signal Transduction

CLC Number:

R318

Wu Zhi-yuan, Shi Yu-cang, Jiang Jun-jian, Wu Zhi-xian, Zhang Hui-jun, Liu Yan-fang, Liu Hong-wei. hIL-24 gene influences the biological characteristics of the keloid by regulating transforming growth factor-beta/Smad pathway[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(33): 4926-4932.

Figures/Tables 3

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