Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (33): 4883-4889.doi: 10.3969/j.issn.2095-4344.2016.33.003

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Effects of drug serum of Jianbuhuqian pills on proliferation and function of osteoblast-like cells in vitro

Luo Yi1, 2, Deng You-zhang2, He Ben-xiang3, Hou Wei-guang1, Ding Xiao-chuan1, Liu Xuan-wen1, Qing Chun1   

  1. 1363 Hospital, Chengdu 610041, Sichuan Province, China; 2Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China; 3Chengdu Kinesiology University, Chengdu 610041, Sichuan Province, China
  • Received:2016-05-29 Online:2016-08-12 Published:2016-08-12
  • Contact: Qing Chun, Master, Master’s supervisor, 363 Hospital, Chengdu 610041, Sichuan Province, China
  • About author:Luo Yi, M.D., Associate chief physician, 363 Hospital, Chengdu 610041, Sichuan Province, China; Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China
  • Supported by:

    the National Sci-Tech Support Plan of China, No. 2012BAK21B01-02-2

Abstract:

BACKGROUND: More recently, the focus has been on searching for a compound Chinese medicine for reinforcing kidney, which cannot only inhibit bone absorption, but also promote osteogenesis to protect against osteoporosis.
OBJECTIVE: To explore effects of drug serum of Jianbuhuqian pills on proliferation and function of osteoblast-like cells in vitro.
METHODS: Twenty-four adult female Sprague-Dawley rats were randomly divided into low-dose, medium-dose, high-dose and normal saline groups, and given intragastric administration of 1.5, 3.0, and 6 g/kg Jianbuhuqian pills and equal volume of normal saline, respectively twice daily for 1 week. At 1 hour after final gavage, rats were decapitated to prepare drug sera used for culturing osteoblast-like cells. At 24, 48 and 72 hours of culture, the cellular morphology was observed, as well as the cell proliferation and alkaline phosphatase activity was detected by MTT assay and alkaline phosphatase staining, respectively.
RESULTS AND CONCLUSION: Compared with the normal saline group, the cell density began to increase significantly in three Jianbuhuqian groups at 24 hours after culture, mitotic figures were easy to be observed, cells were in overlapping growth, much secretions and matrix accumulation appeared, especially in the high-dose group. The obsorbance values in Jianbuhuqian groups were significantly higher than that in the normal saline group. After 24 hours of culture, the obsorbance values in the medium-dose and high-dose groups were significantly increased compared with the low-dose group, and the values showed significant differences among three Jianbuhuqian groups after 48 and 72-hour culture. In addition, the alkaline phosphatase activity presented overt increase in the Jianbuhuqian groups compared with the normal saline group, and significant differences could be found among Jianbuhuqian groups. To conclude, the drug serum of Jianbuhuqian pills can promote the activity of osteoblast-like cells in a time- and concentration-dependent manner.

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

Key words: Serum, Osteoblasts, Alkaline Phosphatase, Tissue Engineering

CLC Number: