Adipose-derived stem cell transplantation for osteoporosis rats: evaluation by bone mineral density and histomorphometry measurement

doi:10.3969/j.issn.2095-4344.2016.32.017

Abstract

Abstract:

BACKGROUND: Stem cell transplantation is increasingly hoped to promote osteoblast differentiation and inhibit osteoclast proliferation in the treatment of osteoporosis.
OBJECTIVE: To study the therapeutic effect of exogenous adipose-derived stem cell (ADSC) transplantation on osteoporosis in ovariectomized rats.
METHODS: Thirty Sprague-Dawley female rats were equivalently randomized into sham, model, ADSC transplantation groups. Rats in all groups except the sham group underwent bilateral ovariectomy to make osteoporosis models. Surrounding adipose tissues instead of the ovary were removed in the sham group. After modeling, rats were given 2×10⁶ ADSCs at passage 4 via the tail vein in the transplantation group and the same volume of normal saline in the model group, once a week. After 6 weeks, levels of serum calcium, phosphorus, and alkaline phosphatase as well as bone mineral density and histomorphometry indicators were detected in rats.
RESULTS AND CONCLUSION: Compared with the sham group, the trabecular bone volume fraction was significantly decreased in the model group (P < 0.01), but remarkably increased after ADSC transplantation (P < 0.05). After modeling, the bone trabecular absorption surface percentage and rate of bone trabecular formation were elevated significantly (P < 0.05 or P < 0.01), while these increases were improved by ADSC transplantation (P < 0.05). Additionally, the levels of serum calcium and alkaline phosphatase and bone mineral density were significantly decreased after modeling, but were increased after ADSC transplantation. In contrast, the serum level of phosphorus was significantly increased in the model group (P < 0.05) but decreased markedly in the ADSC transplantation group (P < 0.05). To conclude, ADSC transplantation can reduce the loss of bone mass in osteoporosis rats by ovariectomy.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Osteoporosis, Adipose Tissue, Stem Cell Transplantation, Tissue Engineering

CLC Number:

R394.2

Li Guang-zhang, Jiang Zhao-qin, Liu Zhi-xin. Adipose-derived stem cell transplantation for osteoporosis rats: evaluation by bone mineral density and histomorphometry measurement[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(32): 4825-4830.

Figures/Tables 3

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