Oligodendrocyte transplantation for acute spinal cord injury: insulin-like growth factor 1 induces bone marrow mesenchymal stem cells differentiating into oligodendrocytes

doi:10.3969/j.issn.2095-4344.2016.23.011

Abstract

Abstract:

BACKGROUND: Treatment and rehabilitation of spinal cord injury is a complicated problem, and the reconstruction and remyelination of neural reflex pathways are the essential process, during which oligodendrocytes play an important role in spinal cord injury repair.
OBJECTIVE: To observe the effect of oligodendrocyte transplantation for acute spinal cord injury in rats.
METHODS: Insulin-like growth factor 1 induced bone marrow mesenchymal stem cells differentiating into oligodendrocytes, and those oligodendrocytes were transplanted into rats with acute spinal cord injury as induced cell transplantation group. Simple normal saline and natural oligodendrocytes were transplanted into the rat injured spinal cord as control group and oligodendrocyte group, respectively. Rat behavioral changes were observed by inclined plane test and Basso-Beattie-Bresnahan (BBB) scores. Neurological recovery and survival of the transplanted cells was detected and observed using spinal evoked potential and immunohistochemical staining, respectively.
RESULTS AND CONCLUSION: Compared with the control group, BBB scores and the critical elevation angle of the incline plane test significantly increased, latencies of spinal motor and sensory evoked potential were on the decline (P < 0.05), and there were no significant differences in above indicators between the two groups at 4 and 8 weeks after transplantation. Moreover, survived oligodendrocytes after transplantation could be found in the lesions of spinal cord in both two groups. In conclusion, insulin-like growth factor 1-induced bone marrow mesenchymal stem cells can differentiate into oligodendrocytes that exact an excellent role in acute spinal cord injury repair after transplantation, which achieve the equal clinical efficacy to the natural oligodendrocytes.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Spinal Cord Injuries, Tissue Engineering

CLC Number:

R394.2

Wei Fen, Xiao Xiu-lan. Oligodendrocyte transplantation for acute spinal cord injury: insulin-like growth factor 1 induces bone marrow mesenchymal stem cells differentiating into oligodendrocytes[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(23): 3419-3424.

Figures/Tables 3

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