Tissue-engineered annular fibrosus scaffold constructed by konjac glucomannan/ nano-hydroxyapatite/collagen composite material

doi:10.3969/j.issn.2095-4344.2016.16.018

Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (16): 2412-2417.doi: 10.3969/j.issn.2095-4344.2016.16.018

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Tissue-engineered annular fibrosus scaffold constructed by konjac glucomannan/ nano-hydroxyapatite/collagen composite material

Zhuang Ying^{1, 2}, Chen Xi-liang³, Li Yong¹, Chen Qing-hua³, Pan Xing-hua², Xu Yong-qing²

1the 59th Hospital of PLA, Minimally Invasive Diagnosis and Treatment Center of Spinal Surgery of Chengdu Military Region, Kaiyuan 661699, Yunnan Province, China; 2Orthopaedics Center of PLA/Research Center of Stem Cell, Tissue and Organ Engineering, Kunming General Hospital of Chengdu Military Region, Kunming 650032, Yunnan Province, China; 3School of Materials Science and Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China

Received:2016-02-10 Online:2016-04-15 Published:2016-04-15
Contact: Xu Yong-qing, Professor, Doctoral supervisor, Orthopaedics Center of PLA/Research Center of Stem Cell, Tissue and Organ Engineering, Kunming General Hospital, Chengdu Military Region, Kunming 650032, Yunnan Province, China
About author:Zhuang Ying, M.D., Attending physician, the 59th Hospital of PLA, Minimally Invasive Diagnosis and Treatment Center of Spinal Surgery of Chengdu Military Region, Kaiyuan 661699, Yunnan Province, China
Supported by:
Chengdu Military Medical Science and Technology Research Project, No. C14013

Abstract

Abstract:

BACKGROUND: Tissue-engineered transplantation technique has become an ideal therapeutic regimen for degenerative disc diseases through reconstituting the biological functions of the degenerated intervertebral discs.

OBJECTIVE: To construct a novel tissue-engineered annular fibrosus scaffold.

METHODS: Konjac glucomannan, nano-hydroxyapatite and collagen were used to fabricate a new tissue-engineered annular fibrosus scaffold by wet spinning, chemical crosslinking, and freeze drying methods. Afterwards, X-ray diffraction and Fourier transform infrared spectrometer were used to analyze the scaffold qualitative components, physico-chemical property, biomechanical performance and cytocompatibility.

RESULTS AND CONCLUSION: The bionic scaffold had a three-dimensional porous structure, with the average pore size of (425.8±47.3) μm, the average porosity of (73.4±5.6)%, and the water absorption of (718.6±24.3)%. In addition, the compressive strength of the scaffold was similar with that of the natural annular fibrosus. More importantly, the scaffold had good biocompatibility without cytotoxicity. These results show that the tissue-engineered annular fibrosus scaffold constructed by konjac glucomannan, nano-hydroxyapatite and collagen has proper three-dimensional porous structure, biocompatibility, porosity, water absorption and biomechanical strength.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Durapatite, Collagen, Tissue Engineering

Zhuang Ying, Chen Xi-liang, Li Yong, Chen Qing-hua, Pan Xing-hua, Xu Yong-qing. Tissue-engineered annular fibrosus scaffold constructed by konjac glucomannan/ nano-hydroxyapatite/collagen composite material[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(16): 2412-2417.

Figures/Tables 3

References

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Tissue-engineered annular fibrosus scaffold constructed by konjac glucomannan/ nano-hydroxyapatite/collagen composite material

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