Biocompatibility of domestic porous tantalum carrying bone morphogenetic protein 7 in the erector spinae muscle of rabbits

doi:10.3969/j.issn.2095-4344.2016.16.013

Abstract

Abstract:

BACKGROUND: Bone morphogenetic protein 7 (BMP-7) can induce bone and cartilage formation in vivo, and induce chondrogenic and osteogenic differentiation of mesenchymal cells in muscles and around the vessels.

OBJECTIVE: To observe the structure of domestic tantalum-muscle interface fibrous capsule, growth of muscle and small blood vessels into the porous tantalum and the ability of ectopic osteogenesis after implantation of porous tantalum loaded with BMP-7 into the erector spinae of rabbits.

METHODS: Porous tantalum slices loaded with BMP-7 (experimental group) and porous tantalum slices (control group) were implanted into the erector spinae muscle of New Zealand white rabbits. And the porous tantalum slices with surrounding muscle tissues about 0.5 cm thick were removed at 2, 4, 8 weeks after implantation, and observed under scanning electron microscope for hematoxylin eosin staining, Masson staining and hard tissue slice observation.

RESULTS AND CONCLUSION: (1) Hematoxylin-eosin staining: Fibrous capsule formation was observed around the materials in the two groups, and with the extension of time, the fibrous capsules were slightly dense, and thinned. There was no obvious inflammatory reaction in the interface between the material and the muscle. There was no significant difference between the two groups in the fibrous capsules thickness. (2) Scanning electron microscope: 2 weeks after the surgery, a small amount of collagen and muscle fibers were formed in the porous tantalum pores in the two groups, and some of collagen fibers attached to the pore walls. At 8 weeks after the surgery, all the pores of porous tantalum were full of muscle fibers that were combined with the pore wall closely. There was no significant difference between the two groups. (3) Hard tissue slices: 2 weeks after the surgery, a small amount of fibroblast cells and muscle fibers grew into the pores of porous tantalum in the two groups and new capillaries grew into the pores of porous tantalum in the experimental group. At 8 weeks after the surgery, the porous tantalum and all the pores were full of muscle fibers that were combined with the pore wall closely, the number of small blood vessels and cells decreased, and the tantalum and the muscle were fused closely. (4) Masson staining: 8 weeks after the surgery, a large number of mesenchymal cells, ossein and cartilage matrix formed in the muscle gaps and a few cartilage bone tissues were formed in the experimental group, but no cartilage was found in the control group. The study showed that porous tantalum carrying BMP-7 has good biocompatibility and osteogenic induction ability.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Tantalum, Bone Morphogenetic Protein 7, Tissue Engineering

Zhang Hui, Wang Qian, Tao Jian-feng, Wang Ai-jun, Shi Wei, Bian Yu-jie, Li Qi-jia, Wang Zhi-qiang. Biocompatibility of domestic porous tantalum carrying bone morphogenetic protein 7 in the erector spinae muscle of rabbits[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(16): 2376-2383.

Figures/Tables 6

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