Different induction methods for bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2015.28.014

Abstract

Abstract:

BACKGROUND: Transformation growth factor beta 1 is mostly used to induce the chondrogenic differentiation of bone marrow mesenchymal stem cells, but there is a poor induction efficacy.

OBJECTIVE: To explore the chondrogenic differentiation of bone marrow mesenchymal stem cells co-cultured with articular chondrocytes or induced by transforming growth factor beta 1.

METHODS: Articular chondrocytes and bone marrow mesenchymal stem cells from SD rats were harvested and divided into 1:2, 2:1, 1:1 concentration groups. Cells induced by transforming growth factor beta 1 acted as control group. After 20 days of induced culture, MTT was used to detect cell viability, alcian blue colorimetric assay was applied to measure glycosaminoglycan content, and western blot assay was employed to determine the expression of collagen type II.

RESULTS AND CONCLUSION: The absorbance value in the control group was significantly lower than that in the 1:1 and 2:1 groups (P < 0.05). Glycosaminoglycan content and protein expression of collagen type II were also lower in the control group than the 1:2, 1:1, 2:1 groups. But there was no difference between 1:1 and 2:1 groups (P > 0.05). The results show that bone marrow mesenchymal stem cells co-cultured with articular chondrocytes can be induced to differentiate into chondrocytes, and meanwhile, there is a saturation phenomenon during the chondrogenic differentiation of bone marrow mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Chondrocytes, Coculture Techniques, Transforming Growth Factor beta1

CLC Number:

R394.2

Dai Chuan-qiang1, Jia Xu-feng2, Zhang Lin3, Zhang Ge4 . Different induction methods for bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(28): 4503-4507.

Figures/Tables 1

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