Biological characteristics of G666-1 nasopharyngeal cancer stem cells in Survivin gene silencing

doi:10.3969/j.issn.2095-4344.2015.28.015

Abstract

Abstract:

BACKGROUND: Survivin gene is the most potent anti-apoptosis factor, which can inhibit tumor cell proliferation and migration. It has become a hot topic related to cancer research in recent years.

OBJECTIVE: To explore the effect of Survivin gene silencing on the biological characteristics of G666-1 nasopharyngeal carcinoma cells.

METHODS: siRNA sequence of Survivin was designed and synthesized and then transfected into G666-1 cells using LipofectamineTM 2000. After Survivin gene silencing, Survivin mRNA and protein expressions were detected by RT-PCR and western blot assay, respectively. Expression of CD44⁺CD133⁺ cells was detected by flow cytometry. In addition, the effects of Survivin gene silencing on the proliferation, migration and invasion of G666-1 cells were evaluated through cell counting kit-8 test, scratch healing assay and cell invasion assay.

RESULTS AND CONCLUSION: After Survivin gene silencing, the expressions of Survivin mRNA and protein were significantly reduced (P < 0.05); the growth of G666-1 cells was slowed dramatically, indicating the proliferation ability was inhibited, and the migration and invasion abilities were both decreased significantly (P < 0.05); the expression ratio of CD44⁺CD133⁺ cells was significantly lowered (P < 0.05). These findings indicate that the proliferation, migration and invasion of G666-1 nasopharyngeal cancer stem cells can be suppressed after Survivin gene silencing, and the proportion of CD44⁺CD133⁺ cells is reduced, thus indicating Survivin gene silencing may be a new effective target for treatment of nasopharyngeal carcinoma.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Nasopharyngeal Neoplasms, Neoplastic Stem Cells, Gene Silencing, Cell Proliferation

CLC Number:

R394.2

Wang Hui. Biological characteristics of G666-1 nasopharyngeal cancer stem cells in Survivin gene silencing[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(28): 4508-4513.

Figures/Tables 6

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