Ultrastructural changes of reconstructed mandibular condylar cartilage under continuous mandibular advancement

doi:10.3969/j.issn.2095-4344.2015.29.009

Abstract

Abstract:

BACKGROUND: Previous animal experiments have demonstrated that mandibular advancement can cause the remodeling of temporomandibular joint tissue of young SD rats. This is mainly characterized by accelerated growth rate of the condyle tissue and secondary growth of mandible. But the ultrastructural remodeling of condylar chondrocytes remains poorly understood.
OBJECTIVE: To observe the histological and ultrastructural variations of reconstructed condylar cartilage of young rats under the effect of continuous mandibular advancement.
METHODS: SD rats aged 4 weeks were randomly divided into control and experimental groups. Rats in the experimental group were subjected to mandibular advancement for 24 hours and sacrificed at 3, 7, 14, 21 and 30 days of intervention. Condylar cartilage samples were harvested and their histological and ultrastructural changes were observed under optical microscope and transmission electron microscope.
RESULTS AND CONCLUSION: After 14 days of intervention, the thickness of condylar cartilage in the experimental group increased first and then became thin in the period of observation. The cartilage thickness variations in the postmedian condylar were significant (P < 0.01). After 7 days of intervention, the ultrastructure
of condylar chondrocytes was reconstructed, including intracellular karyopyknosis, rough endoplasmic reticulum compartment swelling, smaller even absent lipid droplets, less and irregular microfilaments around the nucleus, broadened and increased extracellular matrix and the emergence of large gaps. These results demonstrate that under continuous mandibular advancement, the rat condylar cartilage will become thick or thin with the endurance time, and chondrocyte matrix synthesis ability will be significantly enhanced.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Tissue Engineering, Condyle, Cartilage, Ultrastructure

CLC Number:

R318

Li Xue, Wang Ming-guo, Yang Shuai, Gao Jie, Fan Yuan-yuan, Song Yan. Ultrastructural changes of reconstructed mandibular condylar cartilage under continuous mandibular advancement [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(29): 4634-4639.

Figures/Tables 1

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