Acellular spinal cord scaffold: preparation and biological characteristics

doi:10.3969/j.issn.2095-4344.2015.21.022

Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (21): 3398-3402.doi: 10.3969/j.issn.2095-4344.2015.21.022

Previous Articles Next Articles

Acellular spinal cord scaffold: preparation and biological characteristics

Li Yi-peng^{1, 2}, Chen Xu-yi^{2, 3, 4}, Zhu Xiang^{1, 2}, Lu Lei^{1, 2}, Tu Yue^{2, 3, 4}

¹Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China; ²Tianjin Key Laboratory of Neurological Trauma Repair, Tianjin 300162, China; ³Institute of Traumatic Brain Injury and Neurology, Affiliated Hospital of Logistics University of CAPF, Tianjin 300162, China; ⁴Affiliated Hospital of Logistics College of CAPF, Tianjin 300162, China

Online:2015-05-21 Published:2015-05-21
Contact: Tu Yue, Professor, Master’s supervisor, Tianjin Key Laboratory of Neurological Trauma Repair, Tianjin 300162, China; Institute of Traumatic Brain Injury and Neurology, Affiliated Hospital of Logistics University of CAPF, Tianjin 300162, China; Affiliated Hospital of Logistics College of CAPF, Tianjin 300162, China
About author:Li Yi-peng, Studying for master’s degree, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China; Tianjin Key Laboratory of Neurological Trauma Repair, Tianjin 300162, China Chen Xu-yi, M.D., Attending physician, Master’s supervisor, Tianjin Key Laboratory of Neurological Trauma Repair, Tianjin 300162, China; Institute of Traumatic Brain Injury and Neurology, Affiliated Hospital of Logistics University of CAPF, Tianjin 300162, China; Affiliated Hospital of Logistics College of CAPF, Tianjin 300162, China Li Yi-peng and Chen Xu-yi contributed equally to this work.
Supported by:
the Science and Technology Support Program of Tianjin, No. 14ZCZDGX00500; the National Natural Science Foundation of China, No. 11102235; the Natural Science Foundation of Tianjin, No. 12JCZDJC24100; the Scientific Fund of Tianjin Health Bureau, No. 2013KZ134; the Seed Fund of the Affiliated Hospital of Logistics College of CAPF, No. FYM201432

Abstract

Abstract:

BACKGROUND: Acellular spinal cord matrix material for framework of spinal cord scaffold has been shown to restore the neurologic function of the damaged spinal cord completely or partially.
OBJECTIVE: To introduce the preparation and biological characteristics of acellular matrix scaffold for the spinal cord and to do an overview of its application and progress in spinal cord tissue engineering.
METHODS: A computer-based search of CNKI and PubMed was performed for articles related to acellular spinal cord scaffolds published from January 2005 to October 2014. The keywords were “acellular spinal cord; scaffold; spinal cord injury; tissue engineering” in Chinese and English, appearing in the title and abstract.
RESULTS AND CONCLUSION: The acellular spinal cord scaffold has low antigenicity, excellent biocompatibility and three-dimensional scaffold structure similar to the spinal cord, but it also possesses poor mechanical properties and structural instability. The scaffold modified by genipin and glutaraldehyde as cross-linking agents
can be significantly improved in the biological performance. Currently, there are some explorations on the application of acellular spinal cord scaffold in nerve repair and regeneration, which lays a foundation for spinal cord tissue engineering. As its lots of advantages, the acellular spinal cord matrix material is expected to be an ideal material for spinal cord tissue engineering.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Biomedical Engineering, Spinal Cord Injuries, Tissue Engineering

CLC Number:

R318

Li Yi-peng, Chen Xu-yi, Zhu Xiang, Lu Lei, Tu Yue. Acellular spinal cord scaffold: preparation and biological characteristics[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(21): 3398-3402.

Figures/Tables 2

References

[1] Guo SZ,Ren XJ,Wu B,et al.Preparation of the acellular scaffold of the spinal cord and the study of biocompatibility. Spinal Cord.2010;48(7):576-581.

[2] Weber B,Emmert MY,Schoenauer R,et al.Tissue engineering on ma-trix: future of autologous tissue replacement. Semin Immunopathol.2011;33(3):307-315.

[3] Nishibe T,Kondo Y,Muto A,et a1.Optimal prost hetic graft design for sni&ll diameter vascular grafts.Vascular.2007;(6): 356-360．

[4] Vavken P,Joshi S,Murray MM.TRITON‐X is most effective among three decellularization agents for ACL tissue engineering. J Orthop Res.2009;27(12):1612-1618.

[5] Yu BT, Li WT,Song BQ,et al. Comparative study of the Triton X-100-sodium deoxycholate method and detergent-enzymatic digestion method for decellularization ofporcine aortic valves.Eur Rev Med Pharmacol Sci.2013;17(16):2179-2184.

[6] Cebotari S,Tudorache I,Jaekel T,et al.Detergent decellularization of heart valves for tissue engineering: toxicological effects of residual detergents on human endothelial cells.Artif Organs. 2010;34(3):206-210.

[7] Deeken CR,White AK,Bachman SL,et al.Method of preparing a decellularized porcine tendonusing tributyl phosphate.J Biomed Mater Res B Appl Biomater.2011;96(2):199-206.

[8] 张国安,宁方刚,钟京鸣,等.反复冻融配合超声振荡洗涤制备猪脱细胞真皮基质[J].中国组织工程研究与临床康复,2007,11(41): 8280-8284．

[9] Azhim A,Yamagami K,Muramatsu K,et al.The use of sonication treatment to comletely decellularize aorta tissue.Conf Proc IEEE Eng Med Biol Soc.2011;2011: 2468-2471.

[10] Hopkinson A,Shanmuganathan VA,Gray T,et al. Optimization ofamniotic membran e (AM) denuding for tissue engineering. Tissue Eng Part C Methods.2008;14(4):371-381.

[11] Liu TX,Wang Z.Collagen crosslinking of porcine sclera using genipin.Acta Ophthalmol.2013;91(4):e253-257.

[12] 蒋涛,任先军,阴洪,等.京尼平交联对大鼠脱细胞脊髓生物学特性的影响[J].第三军医大学学报,2013,35(20):2164-2167．

[13] Bi L,Cao Z,Hu Y,et al.Effects of different cross-linking conditions on the properties of genipin-cross-linked chitosan/collagen scaffolds for cartilage tissue engineering. J Mater Sci Mater Med.2011;22(1):51-62.

[14] Yahyouche A,Zhidao X,Czernuszka JT,et al.Macrophage- mediated degradation of crosslinked collagen scaffolds.Acta biomaterialia.2011;7(1):278-286.

[15] 綦惠,杰永生,陈磊,等.两种交联剂制备软骨细胞移植支架的比较[J].中国医药生物技术,2013,8(6):408-413．

[16] 郭树章,任先军,蒋涛,等.脱细胞脊髓天然支架的制备及形态学观察[J].中国矫形外科杂志,2007,15(3):226-228．

[17] Singh P,Schwarzbauer JE.Fibronectin matrix assembly is essential for cell condensation during chondrogenesis.J Cell Sci.2014;127(Pt 20):4420-4428.

[18] Turney SG,Bridgman PC.Laminin stimulates and guides axonal outgrowth via growth cone myosin II activity. Nat Neurosci. 2005;8:717-719.

[19] King VR,Alovskaya A,Wei DY,et al.The use of injectable forms of fibrin and fibronectin to support axonal ingrowth after spinal cord injury. Biomaterials. 2010;31(15):4447-4456.

[20] King VR,Hewazy D,Alovskaya A,et al.The neuroprotective effects of fibronectin mats and fibronectin peptides following spinal cord injury in the rat. Neuroscience. 2010;168(2): 523-530.

[21] Lin CY,Lee YS,Lin VW,et al.Fibronectin inhibits chronic pain development after spinal cord injury.J Neurotrauma.2012; 29(3):589-599.

[22] Xia M, Zhu Y. Fibronectin enhances spinal cord astrocyte proliferation by elevating P2Y1 receptor expression.J Neurosci Res.2014;92(8):1078-1090.

[23] Deng L,Walker C,Wen X,et al. Laminin-coated filaments co-grafted with Schwann cells and GDNF direct axonal growth following rat thoracic spinal cord injury (729.2). FASEB J.2014;28(1 Supplement):729.

[24] Menezes K,Nascimento MA,Gonçalves JP,et al.Human Mesenchymal Cells from Adipose Tissue Deposit Laminin and Promote Regeneration of Injured Spinal Cord in Rats. PloS One.2014;9(5):e96020.

[25] Menezes K,de Menezes JR,Nascimento MA,et al.Polylaminin, a polymeric form of laminin, promotes regeneration after spinal cord injury. FASEB J.2010;24(11): 4513-4522.

[26] Yuan N,Tian W,Sun L,et al.Neural stem cell transplantation in a double-layer collagen membrane with unequal pore sizes for spinal cord injury repair. Neural Regen Res.2014;9(10): 1014.

[27] Sekeljic V,Andjus PR.Tenascin-C and its functions in neuronal plasticity.Int J Biochem Cell Biol.2012;44:825-829.

[28] Tsai HL,Chiu WT,Fang CL,et al.Different forms of tenascin-C with tenascin-R regulate neural differentiation in bone marrow-derived human mesenchymal stemcells.Tissue Eng Part A.2014;20(13-14):1908-1921.

[29] Crapo PM,Medberry CJ,Reing JE,et al.Biologic scaffolds composed of central nervous system extracellular matrix. Biomaterials.2012;33(13):3539-3547.

[30] Liu J,Chen J,Liu B,et al.Acellular spinal cord scaffold seeded with mesenchymal stem cells promotes long-distance axon regeneration and functional recovery in spinal cord injured rats.J Neurol Sci.2013;325(1):127-136.

[31] Chen J,Joon Lee H,Jakovcevski I,et al.The extracellular matrix glycoprotein tenascin-C is beneficial for spinal cord regeneration.Mol Ther.2010;18(10):1769-1777.

[32] Crapo PM,Medberry CJ,Reing JE,et al.Biologic scaffolds composed of central nervous system extracellular matrix. Biomaterials.2012;33(13):3539-3547.

Acellular spinal cord scaffold: preparation and biological characteristics

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 2

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Zhang Tongtong, Wang Zhonghua, Wen Jie, Song Yuxin, Liu Lin. Application of three-dimensional printing model in surgical resection and reconstruction of cervical tumor [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(9): 1335-1339.
[2]	Zeng Yanhua, Hao Yanlei. In vitro culture and purification of Schwann cells: a systematic review [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(7): 1135-1141.
[3]	Xu Dongzi, Zhang Ting, Ouyang Zhaolian. The global competitive situation of cardiac tissue engineering based on patent analysis [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(5): 807-812.
[4]	Wu Zijian, Hu Zhaoduan, Xie Youqiong, Wang Feng, Li Jia, Li Bocun, Cai Guowei, Peng Rui. Three-dimensional printing technology and bone tissue engineering research: literature metrology and visual analysis of research hotspots [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(4): 564-569.
[5]	Chang Wenliao, Zhao Jie, Sun Xiaoliang, Wang Kun, Wu Guofeng, Zhou Jian, Li Shuxiang, Sun Han. Material selection, theoretical design and biomimetic function of artificial periosteum [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(4): 600-606.
[6]	Liu Fei, Cui Yutao, Liu He. Advantages and problems of local antibiotic delivery system in the treatment of osteomyelitis [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(4): 614-620.
[7]	Li Xiaozhuang, Duan Hao, Wang Weizhou, Tang Zhihong, Wang Yanghao, He Fei. Application of bone tissue engineering materials in the treatment of bone defect diseases in vivo [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(4): 626-631.
[8]	Zhang Zhenkun, Li Zhe, Li Ya, Wang Yingying, Wang Yaping, Zhou Xinkui, Ma Shanshan, Guan Fangxia. Application of alginate based hydrogels/dressings in wound healing: sustained, dynamic and sequential release [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(4): 638-643.
[9]	Chen Jiana, Qiu Yanling, Nie Minhai, Liu Xuqian. Tissue engineering scaffolds in repairing oral and maxillofacial soft tissue defects [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(4): 644-650.
[10]	Xing Hao, Zhang Yonghong, Wang Dong. Advantages and disadvantages of repairing large-segment bone defect [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(3): 426-430.
[11]	Chen Siqi, Xian Debin, Xu Rongsheng, Qin Zhongjie, Zhang Lei, Xia Delin. Effects of bone marrow mesenchymal stem cells and human umbilical vein endothelial cells combined with hydroxyapatite-tricalcium phosphate scaffolds on early angiogenesis in skull defect repair in rats [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(22): 3458-3465.
[12]	Wang Hao, Chen Mingxue, Li Junkang, Luo Xujiang, Peng Liqing, Li Huo, Huang Bo, Tian Guangzhao, Liu Shuyun, Sui Xiang, Huang Jingxiang, Guo Quanyi, Lu Xiaobo. Decellularized porcine skin matrix for tissue-engineered meniscus scaffold [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(22): 3473-3478.
[13]	Mo Jianling, He Shaoru, Feng Bowen, Jian Minqiao, Zhang Xiaohui, Liu Caisheng, Liang Yijing, Liu Yumei, Chen Liang, Zhou Haiyu, Liu Yanhui. Forming prevascularized cell sheets and the expression of angiogenesis-related factors [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(22): 3479-3486.
[14]	Liu Chang, Li Datong, Liu Yuan, Kong Lingbo, Guo Rui, Yang Lixue, Hao Dingjun, He Baorong. Poor efficacy after vertebral augmentation surgery of acute symptomatic thoracolumbar osteoporotic compression fracture: relationship with bone cement, bone mineral density, and adjacent fractures [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(22): 3510-3516.
[15]	Liu Liyong, Zhou Lei. Research and development status and development trend of hydrogel in tissue engineering based on patent information [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(22): 3527-3533.