Migration, homing and differentiation of endogenous bone marrow mesenchymal stem cells after myocardial infarction

doi:10.3969/j.issn.2095-4344.2015.19.005

Abstract

Abstract:

BACKGROUND: Most of studies have focused on the transplantation and regeneration of exogenous stem cells-derived myocardial cells after myocardial infarction, but relatively few studies address the migration, homing and differentiation of endogenous stem cells.
OBJECTIVE: To observe the migration, homing and differentiation of endogenous bone marrow mesenchymal stem cells after myocardial infarction.
METHODS: Adult female C57BL/6 mice were randomized into two groups: myocardial infarction group (n=4), in which, the left anterior descending branch of the coronary artery was ligated to establish acute myocardial infarction models at 4 weeks after bone marrow remodeling, and 1 week later, the model mice were sacrificed; control group (n=3), in which, the mice were subject to bone marrow remodeling and then sacrificed after 4 weeks. Heart tissues were extracted from two groups followed by immunofluorescent detection of Troponin I expression, and the distribution and differentiation of bone marrow mesenchymal stem cells positive for green fluorescent protein after myocardial infarction were observed.
RESULTS AND CONCLUSION: After histological and immunohistochemical examination, bone marrow
mesenchymal stem cells positive for green fluorescent protein were observed in both myocardial infarction group and control group, while more positive cells were seen in the myocardial infarction group. Some bone marrow mesenchymal stem cells in both two groups were positive for green fluorescent protein, Troponin I and PI. However, more triple-positive cells were observed in the myocardial infarction group compared to the control group. Endogenous bone marrow mesenchymal stem cells can migrate, home to the damage myocardium and acquire cardiogenic phenotype after myocardial infarction.

Key words: Bone Marrow, Mesenchymal Stem Cells, Myocardial Infarction, Cell Differentiation

CLC Number:

R394.2

He Xiao-qing, Lin Yu-hui, Huang Shu-ling, Dai Wen-jun, Xu Yun-hong, Chen You-quan, Chen Xi-ming, Chen Min-sheng. Migration, homing and differentiation of endogenous bone marrow mesenchymal stem cells after myocardial infarction [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(19): 2981-2985.

Figures/Tables 2

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