Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (25): 3994-4000.doi: 10.12307/2021.010

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Umbilical cord mesenchymal stem cell transplantation for traumatic systemic inflammatory response syndrome in tree shrews

Ruan Guangping1, 2, 3, Yao Xiang1, 2, 3, Liu-Gao Miyang1, 2, 3, Cai Xuemin1, 2, 3, Li Zian1, 2, 3, Pang Rongqing1, 2, 3, Wang Jinxiang1, 2, 3, #br# Pan Xinghua1, 2, 3#br#   

  1. 1Basic Medical Laboratory of 920 Hospital of Joint Logistics Support Force of PLA, Kunming 650032, Yunnan Province, China; 2Integrated Engineering Laboratory of Cell Biological Medicine of State and Regions, Kunming 650032, Yunnan Province, China; 3Transfer Medicine Key Laboratory of Cell Therapy Technology of Yunan Province, Kunming 650032, Yunnan Province, China
  • Received:2020-06-05 Revised:2020-06-06 Accepted:2020-07-29 Online:2021-09-08 Published:2021-03-25
  • Contact: Pan Xinghua, MD, Chief physician, Basic Medical Laboratory of 920 Hospital of Joint Logistics Support Force of PLA, Kunming 650032, Yunnan Province, China; Integrated Engineering Laboratory of Cell Biological Medicine of State and Regions, Kunming 650032, Yunnan Province, China; Transfer Medicine Key Laboratory of Cell Therapy Technology of Yunan Province, Kunming 650032, Yunnan Province, China
  • About author:Ruan Guangping, MD, Associate chief physician, Basic Medical Laboratory of 920 Hospital of Joint Logistics Support Force of PLA, Kunming 650032, Yunnan Province, China; Integrated Engineering Laboratory of Cell Biological Medicine of State and Regions, Kunming 650032, Yunnan Province, China; Transfer Medicine Key Laboratory of Cell Therapy Technology of Yunan Province, Kunming 650032, Yunnan Province, China
  • Supported by:
    the Yunnan Science and Technology Plan Project Major Science and Technology Project, No. 2018ZF007 (to PXH); the Yunnan Province Applied Basic Research Program Key Project, No. 2018FA041 (to PXH), No. 2017FA040 (to RGP); National Natural Science Foundation of China, No. 31970515 (to PRQ); 920 Hospital of the PLA Joint Logistics Support Force In-Hospital Technology Plan, No. 2019YGB17 (to RGP), No. 2019YGA05 (to PRQ); The Whole Army Experimental Animal Special Project, No. SYDW[2016]004 (to LGMY).

Abstract: BACKGROUND: The research on the prevention and treatment of war trauma has always been the core content in the field of military medicine. It is of great military and scientific significance to establish repeatable animal models of war traumatic infection, systemic inflammatory response syndrome, shock, multiple organ failure and new treatment methods based on the animal model. 
OBJECTIVE: To investigate the efficacy of umbilical cord mesenchymal stem cells in the treatment of traumatic systemic inflammatory response syndrome. 
METHODS:  Totally 40 out of 50 tree shrews were used to establish the model of traumatic systemic inflammatory response syndrome by intravenous injection of lipopolysaccharide after unilateral femoral comminuted fracture using the impact method. The other 10 tree shrews were used as normal controls. After the model was established for 10 days, umbilical cord mesenchymal stem cells labeled with GFP were transfused through tail vein of 20 tree shrews, and 18 were not reinfused as model control group. The distribution of GFP-labeled cells in vivo was measured in each group at 2 and 10 days after GFP-labeled UCMSCs injection. At 20 days after cell transplantation, samples were taken to observe the pathological changes of each tissue, and peripheral venous blood samples were taken to examine the changes in liver function, renal function and heart function.   
RESULTS AND CONCLUSION: (1) GFP-positive cell distribution was observed in all tissues at 2 and 10 days after cell transplantation. The distribution of GFP-positive cells in pancreatic tissue was more obvious at 2 days after transplantation, and more GFP-positive cells were found in liver tissue 10 days after transplantation. (2) At 20 days after transplantation, hematoxylin eosin staining results showed that cell necrosis and inflammatory cell infiltration were observed in each organ of the model control group, and the treatment group was close to the normal control group. The results of liver function, renal function and heart function in the treatment group returned to normal. (3) These results indicate that umbilical cord mesenchymal stem cells have a certain effect on the treatment of traumatic systemic inflammatory response syndrome, and this method provides a new technical solution for treatment of modern weapon trauma.

Key words: stem cells, umbilical cord mesenchymal stem cells, systemic inflammatory response syndrome, model, tree shrew, transplantation, curative effect, mechanism

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