Knockdown of foxM1 strengthens osteogenic differentiation ability of bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.1564

Abstract

Abstract:

BACKGROUND: FoxM1 is thought to be involved in the regulation of stem cell differentiation fate, but its effect on osteogenic differentiation of mesenchymal stem cells has not been reported. The research on foxM1 gene is mainly focused on the tumor field.
OBJECTIVE: To investigate the effect of shRNA-mediated knockdown of foxM1 on the osteogenic differentiation ability of bone marrow mesenchymal stem cells.
METHODS: Bone marrow mesenchymal stem cells from Sprague-Dawley rats were isolated and cultured by the whole bone marrow adherence method. Recombinant lentivirus carrying foxM1-speci?c shRNA and puromycin-resistance gene was constructed and transfected into the rat bone marrow mesenchymal stem cells, and foxM1-knockdown bone marrow mesenchymal stem cells were acquired after puromycin screening. Alizarin red staining was performed to investigate the osteogenic differentiation ability of bone marrow mesenchymal stem cells cultured in osteogenic differentiation medium. Expression levels of several osteogenesis-related genes were examined using quantitative PCR. The nuclear expression of β-catenin was detected using western blot.
RESULTS AND CONCLUSION: Knockdown of foxM1 in bone marrow mesenchymal stem cells enhanced the capability to form mineralized nodules and significantly increased the mRNA expressions of col1a1 and runx2, while no significant difference was found in the nuclear protein expressions of β-catenin. These results suggest that knockdown of foxM1 can promote the osteogenic differentiation of bone marrow mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Forkhead Transcription Factors, RNA Interference, Bone Marrow, Mesenchymal Stem Cells, Cell Differentiation, Osteoblasts, Tissue Engineering

CLC Number:

Chen Qingui, Zeng Mian, He Wanmei, Zhang Lishan, Zheng Haichong. Knockdown of foxM1 strengthens osteogenic differentiation ability of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(5): 673-679.

Figures/Tables 1

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