Alteration and significance of E-selectin and P-selectin in the tissues surrounding fracture in mouse models

doi:10.3969/j.issn.2095-4344.0603

Abstract

Abstract:

BACKGROUND: E-selectin and P-selectin are important adhesion molecules involved in the inflammatory response in vivo, and inflammatory response plays a positive role in promoting early fracture healing.
OBJECTIVE: To investigate the alterations and significance of E-selectin and P-selectin in the tissues surrounding fracture of mice.
METHODS: Thirty-six BALB/c mice were selected for establishing stable tibia fracture model. The mouse models were randomly divided into 1-, 6-, 12-, 24-, and 48-hour and 3-day groups. The hematoma tissue surrounding the fracture was removed at corresponding time, and contralateral tibial tissues at the healthy side were used as controls. The concentrations of E-selectin and P-selectin in tissues were detected by ELISA.
RESULTS AND CONCLUSION: The concentrations of E-selectin and P-selectin in the tissues surrounding the fracture were significantly higher than those in the healthy side (P < 0.01). E-selectin reached a peak at about 6 hours. P-selectin was the highest at about 24 hours. In summary, the concentrations of E-selectin and P-selectin in the tissues surrounding the fracture were significantly up-regulated, suggesting that E-selectin and P-selectin may mediate the migration and adhesion of inflammatory cells during the early stage of mouse fracture and play an important role in the pathological process of bone repair.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Fractures, Bone, E-Selectin, P-Selectin, Tissue Engineering

CLC Number:

R446

Luo Wei1, Li Xianghe1, Yang Xianteng2, Li Senlei2, Zhang Jin2, Tian Xiaobin2, Sun Li2. Alteration and significance of E-selectin and P-selectin in the tissues surrounding fracture in mouse models[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(3): 404-408.

Figures/Tables 5

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