Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (29): 4735-4742.doi: 10.3969/j.issn.2095-4344.1781

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In vitro embryo culture is the core of embryo engineering technology  

Li Nan, Lin Zhong   

  1. Reproductive Medicine Center, Maternal and Child Health Hospital of Liuzhou City, Liuzhou 545001, Guangxi Zhuang Autonomous Region, China
  • Revised:2019-04-02 Online:2019-10-18 Published:2019-10-18
  • Contact: Lin Zhong, MD, Chief physician, Reproductive Medicine Center, Maternal and Child Health Hospital of Liuzhou City, Liuzhou 545001, Guangxi Zhuang Autonomous Region, China
  • About author:Li Nan, MD, Associate researcher, Reproductive Medicine Center, Maternal and Child Health Hospital of Liuzhou City, Liuzhou 545001, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 2015GXNSFBA139177 (to LN), 2017GXNSFAA198193 (to LZ) and 2017GXNSFAA198199 (to LN); the Self-Financing Research Project of the Health and Family Planning Commission of Guangxi Zhuang Autonomous Region, No. Z20180027 (to LN); Liuzhou Science and Technology Development Plan, No. 2016G020217 (to LN)

Abstract:

BACKGROUND: In vitro embryo culture is one of the key links to animal embryo engineering technology.
OBJECTIVE: To review the recent progress in embryonic development and embryo culture in vitro.
METHODS: PubMed database was searched for relevant articles published from January 1990 to October 2018. The keywords were “embryo engineering; pre-implantation embryo; in vitro embryo culture; assisted reproductive technology, ART” in English. Finally, 76 eligible articles were included in result analysis.
RESULTS AND CONCLUSION: In vitro embryo culture system is a key technology of animal embryo engineering that is used in strict accordance with the medical ethical requirements. This culture system is essential for the smooth production of in vitro embryos using embryonic biotechnology. An improved in vitro embryo culture system is conductive to establishing an in vitro culture environment being closer to the internal environment, thereby enhancing the quality of embryos.

Key words: embryo engineering, in vitro embryo culture, assisted reproductive technology, pre-implantation embryo, culture system, nutrients, developmental block, growth factor

CLC Number: