Intervention with autologous adipose-derived mesenchymal stem cells for immune function in mice with systemic lupus erythematosus

doi:10.3969/j.issn.2095-4344.1708

Abstract

Abstract:

BACKGROUND: Although mesenchymal stem cells have achieved clinical efficacy in the treatment of systemic lupus erythematosus, the endogenous function and immunomodulatory mechanism of the cells need to be further studied.
OBJECTIVE: To investigate the effect of autologous adipose-derived mesenchymal stem cells on immune function in mice with systemic lupus erythematosus and its possible mechanism.
METHODS: Thirty-six MRL/lpr mice were randomly divided into three groups: model control group without treatment, PBS control group with tail vein injection of PBS, and stem cell transplantation group with tail vein injection of autologous adipose-derived mesenchymal stem cells. Another 12 C57BL/6 mice were taken as normal control group.
RESULTS AND CONCLUSION: (1) The cells obtained from autologous adipose tissue of MRL/lpr mice had the characteristics of adipose-derived mesenchymal stem cells. (2) The levels of serum urea nitrogen, creatinine and anti-double-stranded DNA antibodies in the model control group, PBS control group and stem cell transplantation group were higher than those in the normal control group (P < 0.01). The levels of serum urea nitrogen, creatinine and anti-double-stranded DNA antibody in the stem cell transplantation group were lower than those in the model control group and PBS control group (P < 0.05). (3) The positive rates of peripheral blood regulatory T cells in the model control group and PBS control group were lower than those in the normal control group and stem cell transplantation group, and the positive rate of peripheral blood regulatory T cells in the stem cell transplantation group was lower than that in the normal control group (P < 0.01). (4) The positive rates of splenic dendritic cells in the model control group and PBS control group were lower than those in the normal control group and stem cell transplantation group (P < 0.01). (5) Th2 ratio in the model control group, PBS control group and stem cell transplantation group was higher than that in the normal control group (P < 0.05). Th1 ratio and Th1/Th2 ratio in the model control group and PBS control group were lower than those in the normal control group and stem cell transplantation group (P < 0.05). (6) Compared with the normal control group, the levels of interleukin-2, interferon-γ, interleukin-10 and lymphotoxin in the peripheral blood of model control group and PBS control group decreased, while the level of interleukin-4 increased (P < 0.05). Compared with the model control group and PBS control group, the levels of interleukin-2, interleukin-10, interferon-γ and lymphotoxin in the peripheral blood of the stem cell transplantation group increased, while the level of interleukin-4 decreased (P < 0.05). In conclusion, adipose-derived mesenchymal stem cell transplantation can exert effects on the immune function of mice with systemic lupus erythematosus by increasing the proportions of dendritic cells and regulatory T cells and promoting the transformation of helper T lymphocyte subsets.

Key words: adipose-derived mesenchymal stem cells, systemic lupus erythematosus, stem cell transplantation, tail vein injection, tissue engineering, mouse, spleen, regulatory T cells, dendritic cells, helper T cells

CLC Number:

R457
R363

Ye Ling, Zhu Jing, He Chengsong. Intervention with autologous adipose-derived mesenchymal stem cells for immune function in mice with systemic lupus erythematosus[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(17): 2696-2702.

Figures/Tables 4

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