Optimal temperature and storage time span for cryopreservation of platelets

doi:10.3969/j.issn.2095-4344.0642

Abstract

Abstract:

BACKGROUND: Clinical demand of fresh liquid platelets cannot be guaranteed because of the rapid increase of the need of platelets, especially for the specific blood types, emergency patients and remote areas.
OBJECTIVE: To explore the optimal temperature and storage time span for deep cryopreservation of platelets in 5% dimethyl sulfoxide as well as the optimal usage time after melting of the frozen platelets.
METHODS: Sixteen platelet samples filtered by the machine were randomly selected. Indexes as blood platelet count, mean platelet volume, platelet distribution width, CD42b, CD62p were separately tested before and 1, 3, 6, 12 months after cryopreservation at three different temperatures of -18, -40, and -80 ℃ as well as immediately, 1, 2, 4 hours after melting.
RESULTS AND CONCLUSION: Significant changes were found in the expression of CD62p in the platelets at 6 and 12 months after cryopreservation at -80 ℃ and the expression of CD42b in the platelets at 6 months after cryopreservation at -80 ℃ (P < 0.05). The expression of CD42b and CD62p showed a significant difference in the platelets stored at -80 ℃ and at -18 and -40 ℃ for 6 months (P < 0.05). For the platelets stored at -80 ℃ for 6 months, the expression of CD42b and CD62p in the platelets at 4 hours after melting was significantly different from that immediately after melting (P < 0.05). These findings reveal that the lower temperature indicates the better cryopreservation of platelets using 5% dimethyl sulfoxide. The recommended storage conditions are -80 ℃ for 6 months; and the optimal usage time after melting is within 2 hours.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Blood Platelets, Cryopreservation, Dimethyl Sulfoxide, Tissue Engineering

CLC Number:

R394.2

Yang Zhen-yu, Li Zheng-fa, Fu Ling-mei, Zhu Xiang-ming. Optimal temperature and storage time span for cryopreservation of platelets[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(33): 5368-5372.

Figures/Tables 3

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