Nicotinamide N-methyl transferase gene polymorphism and its correlation with the athletic ability of handball players

doi:10.3969/j.issn.2095-4344.0321

Abstract

Abstract:

BACKGROUND: Studies confirm that niacinamide N-methyl transferase gene (NNMT) polymorphism is associated with human body aerobic exercise capacity. Handball is a kind of mixed oxygen sports, accounting for a proportion of anaerobic exercise in competition or peacetime training, mainly in aerobic exercise.
OBJECTIVE: To explore a NNMT gene single nucleotide polymorphism locus rs2256292 associated with the athletic ability of handball players.
METHODS: The NNMT genetic polymorphism locus rs2256292 in 28 athletes from Chinese national male handball team (experimental group) and 53 male students (control group) was tested by polymerase chain reaction-ligase detection reaction. The relative VO2max and body constituents index of skeletal muscle, body fat percentage were also tested.
RESULTS AND CONCLUSION: Frequencies of G allele and GG genotype in the experimental group were significantly higher than those in the control group (P < 0.05). Among the 28 athletes, the relative VO2max in the GG genotype group was higher than that in the CG genotype group (P < 0.05). Among the 53 general students, there were no differences in relative VO2max among genotypes. Among the 28 athletes, the skeletal muscle percentage in the GG genotype group was significantly higher than that in the CC and CG genotype groups (P < 0.05). Among the 53 general students, the skeletal muscle percentage in the GG genotype group was significantly higher than that in the CG genotype group (P < 0.05). In addition, there were no differences in the body fat percentage among genotypes in athletes or general students. These results suggest that the NNMT genetic polymorphism locus rs2256292 is significantly correlated with the athletic ability of handball players, with the athletes being more likely to have G allele and carriers of GG genotype having stronger athletic ability.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Athletes, Body Composition, Polymorphism, Single Nucleotide, Tissue Engineering

CLC Number:

R318

Chen Wei1, Li Jiang-hua2, Gu Jian-ren1, Chen Feng1, Xia Hua1, Zhang Bin1, Jiao Qi-bin1, Zheng Feng3. Nicotinamide N-methyl transferase gene polymorphism and its correlation with the athletic ability of handball players[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(28): 4544-4549.

Figures/Tables 1

2.1 参与者数量分析 81名受试者均完成测试，全部进入结果分析。 2.2 两组rs2256292位点等位基因和基因型频率分布比较如表2所示，实验组28名手球运动员等位基因频率：C为25%，G为75%，基因型频率为CC为10.7%，CG为28.6%，GG为60.7%；对照组53名普通男学生等位基因频率：C为41.5%，G为58.5%，基因型频率CC为15.1%，CG为52.8%，GG为32.1%，经卡方检验符合H-W平衡(P > 0.05)，两组之间的等位基因频率和基因型频率分布均出现了显著差异(P < 0.05)，实验组G等位基因和GG基因型频率显著高于对照组。 2.3 两组rs2256292位点不同基因型之间相对VO2 max的比较如图3所示，28名手球运动员中，基因型CC组相对VO2 max为(59.24±6.69) mL/(kg•min)，基因型GG组相对 VO2 max为(63.39±7.59) mL/(kg•min)，基因型CG组相对 VO2 max为(55.43±8.74) mL/ (kg•min)，基因型GG组与基因型CG组de 相对VO2 max比较差异有显著性意义(P=0.029)；53名普通男学生中，基因型CC组相对VO2 max为(42.12± 3.46) mL/(kg•min)，基因型GG组相对VO2 max为(43.48± 3.07) mL/(kg•min)，基因型CG组相对VO2 max为(42.94± 2.76) mL/(kg•min)，各基因型之间相对VO2 max比较差异无显著性意义(P > 0.05)。以上结果表明，rs2256292位点与相对VO2 max存在显著的关联性，从均值看GG型最好。 2.4 两组rs2256292位点不同基因型之间骨骼肌百分比、体脂百分比的比较如图4所示，28名手球运动员中，基因型CC组、基因型GG组和基因型CG组骨骼肌百分比分别为40.55%，44.03%，41.46%，基因型CC组与基因型GG组(P=0.039)、基因型GG组与基因型CG组(P=0.014)骨骼肌百分比比较差异有显著性意义；基因型CC组、基因型GG组和基因型CG组体脂百分比分别为13.17%，12.03%，11.74%，各基因型间体脂百分比比较差异无显著性意义 (P > 0.05)。 53名普通男学生中，基因型CC组、基因型GG组和基因型CG组骨骼肌百分比分别为35.41%，36.88%，35.07%，基因型GG组与基因型CG组骨骼肌百分比比较差异有显著性意义(P=0.011)；基因型CC组、基因型GG组和基因型CG组体脂百分比分别为15.68%，15.19%，14.7%，各基因型体脂百分比比较差异无显著性意义(P > 0.05)。以上结果表明该多态性位点与机体骨骼肌百分比也存在关联，也是GG型最好；体脂百分比与rs2256292位点关联性不大。"

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