Tissue-engineered bone with vascular bundles or sensory nerve tracts for repairing large bone defects

doi:10.3969/j.issn.2095-4344.2017.08.003

Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (8): 1161-1166.doi: 10.3969/j.issn.2095-4344.2017.08.003

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Tissue-engineered bone with vascular bundles or sensory nerve tracts for repairing large bone defects

Qin Jun-jun1, Yin Dong1, Pei Guo-xian2, Jiang Shan3, Chen Si-yuan4, Wang Le5, Mu Tian-wang6, Li Ming-dong7

1Department of Orthopedics, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, Guangxi Zhuang Autonomous Region, China; 2Department of Orthopedics, Xijing Hospital of the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China; 3Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China; 4Department of Orthopedics, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, Guangdong Province, China; 5Department of Orthopaedics, the Third Affiliated Hospital of Guangzhou University, Guangzhou 510150, Guangdong Province, China; 6Department of Orthopaedics, the Third People’s Hospital of Shenzhen, Shenzhen 518116, Guangdong Province, China; 7Department of Traumatic Orthopedics, Hainan General Hospital, Haikou 570311, Hainan Province, China

Received:2017-01-06 Online:2017-03-18 Published:2017-04-14
Contact: Li Ming-dong, Master, Attending physician, Department of Traumatic Orthopedics, Hainan General Hospital, Haikou 570311, Hainan Province, China
About author:Qin Jun-jun, Master, Attending physician, Department of Orthopedics, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, Guangxi Zhuang Autonomous Region, China、
Supported by:
the National Natural Science Foundation of China, No. 30600643

Abstract

Abstract:

BACKGROUND: Neuropeptides, a kind of endogenous active substance in nerve tissues, can modulate physiological functions of multiple body systems.
OBJECTIVE: To observe the effects of vascular bundles or sensory nerve tracts implanted into tissue-engineered bone for rabbit large bone defects on the expression levels of calcitonin gene-related peptide (CGRP) and neuropeptide-Y.
METHODS: Fifty-four New Zealand rabbits were enrolled to make model of large bone defects, and then, the animal models were randomly divided into three groups, including sensory nerve tract, vascular bundle, and control groups (n=18 per group), followed by implanted with sensory nerve tracts, vascular bundle, and tissue-engineered bone without sensory tracts or vascular bundle, respectively. The defected bone received gross and Masson staining at 4, 8 and 12 weeks after modeling, to compare the expression levels of CGRP and neuropeptide-Y in each group.
RESULTS AND CONCLUSION: mRNA expression levels of CGRP and neuropeptide-Y in the sensory nerve tract and vascular bundle groups were significantly higher than those in the control group at different time points after modeling (P < 0.05). mRNA expression levels of CGRP and neuropeptide-Y in the tissue-engineered bone began to be increased and peaked at the 8th week, and then decreased (P < 0.05), which were the lowest at the 4th week (P < 0.05). Immunohistochemical staining results showed that CGRP was mainly found in the bridge, periosteum of newly born bones and around blood vessels; while neuropeptide-Y mainly localized in the medullary cavity and around blood vessels. These results indicate that the implantation of vascular bundle and sensory nerve tracts for bone defects can upregulate the expression levels of CGRP and neuropeptide-Y, and promote bone repair. However, sensory tract implantation may cause sensory impairment; thereafter, vascular bundle implantation is more suitable for ideal tissue-engineered construction to meet physical requirements.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Calcitonin Gene-Related Peptide, Neuropeptide Y, Tissue Engineering

CLC Number:

R318

Qin Jun-jun1, Yin Dong1, Pei Guo-xian2, Jiang Shan3, Chen Si-yuan4, Wang Le5, Mu Tian-wang6, Li Ming-dong7 . Tissue-engineered bone with vascular bundles or sensory nerve tracts for repairing large bone defects[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(8): 1161-1166.

Figures/Tables 2

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Tissue-engineered bone with vascular bundles or sensory nerve tracts for repairing large bone defects

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