Clinical monitoring of chromosome karyotype and fusion gene expression after allogeneic hematopoietic stem cell transplantation in chronic myelocytic leukemia

doi:10.3969/j.issn.2095-4344.2017.29.017

Abstract

Abstract:

BACKGROUND: It has been reported that 70% of patients with chronic myeloid leukemia (CML) are negative for cytogenetic and genetic markers within 1-5 months after allogeneic hematopoietic stem cell transplantation (allo-HSCT), but there are still some patients who have repeatedly varied outcomes in cytogenetic and genetic marker detection. Overall, the negative rate is up to 89.5% at 3-12 months after allo-HSCT.
OBJECTIVE: To monitor the changes in cytogenetic and genetic marker expression and to explore the prognostic significance in CML patients undergoing allo-HSCT.
METHODS: Seventeen CML patients who had undergone allo-HSCT were enrolled. Chromosome G banding pattern of the bone marrow from these patients were analyzed using short-term culture method and direct method at 30 days, 2, 3, 4, 6, 12, 24, 36, 48, 60, 72 months after allo-HSCT. Dual-color fluorescence in situ hybridization was used to detect bcr-abl fusion gene; bcr-abl expressions in primary bone marrow cells from CML patients were detected using RQ-PCR.
RESULTS AND CONCLUSION: There were 8/17 cases of male patient/male donor and 7/17cases of male patient/female donor (compatriots). 46XX karyotype (women) was detected by multiple reexaminations after transplantation, and there was no Y chromosome or other aberration of chromosome karyotype in their karyotype. Among the 17 cases, 1 case of female patient/female donor (compatriots) and 1 case of female patient/male donor (unrelated) manifested 46 XY chromosome karyotype and bcr-abl positive at 1 month after transplantation; after 4 months, these two cases still maintained 46 XY chromosome karyotype but bcr-abl negative; after 4-96 months, the karyotype continued to remain as 46 XY, and bcr-abl (-). Among the 17 cases, 1 case of male patient/male donor of full-matched compatriot (brother) manifested that Ph chromosomal bcr-abl gene continuously expressed within 1-12 months after allo-HSCT; then the cases was given donor lymphocyte infusion, and the bcr-abl expression returned to be negative at 48 months after transplantation. To conclude, chromosomal karyotype analysis and bcr-abl fusion gene monitoring provide important reference value for subsequent treatment options and prognosis judgment for CML patients with allo-HSCT.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Hematopoietic Stem Cell Transplantation, Leukemia, Myelogenous, Chronic, BCR-ABL Positive, Tissue Engineering

CLC Number:

R394.2

Li Zheng-fa, Liu Wei, Du Yun-yun, Yang Tong-hua, Zhao Jie, Shi Ke-qian, Tang Xin-hua, Yang Yan-mei,Zhang Yin-hong, Lai Xun, Wen Yan, Lu Zhi-xiang. Clinical monitoring of chromosome karyotype and fusion gene expression after allogeneic hematopoietic stem cell transplantation in chronic myelocytic leukemia[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(29): 4691-4696.

Figures/Tables 3

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