Influence of angiogenesis on neural stem cell proliferation in the subventricular zone after focal cerebral ischemia/reperfusion

doi:10.3969/j.issn.2095-4344.2017.29.018

Abstract

Abstract:

BACKGROUND: The angiogenesis may be related to the proliferation of neural stem cells, but there is still no unified view.
OBJECTIVE: To observe the influence of angiogenesis on neural stem cell proliferation in the subventricular zone of rats after focal cerebral ischemia/reperfusion.
METHODS: Male Sprague-Dawley rats were randomly divided into normal group, sham group, vascular endothelial growth factor (VEGF)+cerebral ischemia/reperfusion group, normal saline (NS)+cerebral ischemia/reperfusion group. The injection was done via the lateral cerebral ventricle. Then, each group was subdivided into four groups (1, 2, 7, 14 days after ischemia/reperfusion). Focal cerebral ischemia/reperfusion models were made by the thread method. After modeling, the corresponding intervention was given in each group. The expression changes of Nestin and vWF mRNA in the subventricular zone were detected in all groups by immunohistochemical staining and real-time PCR, respectively.
RESULTS AND CONCLUSION: There was a certain increase in vWF and Nestin positive expression in the subventricular zone after cerebral ischemia/reperfusion. At 7 days after ischemia, the expression of vWF mRNA and Nestin reached the peak, indicating the proliferation of neural stem cells in the subventricular zone after cerebral ischemia/reperfusion is associated with the time of angiogenesis. In addition, the expression of vWF mRNA and Nestin was significantly higher in the VEGF+cerebral ischemia/reperfusion group than the other two groups, indicating angiogenesis could promote the proliferation of neural stem cells in the subventricular zone of rats after cerebral ischemia/reperfusion.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Neural Stem Cells, Brain Ischemia, Vascular Endothelial Growth Factors, Cell Proliferation, Tissue Engineering

CLC Number:

R394.2

Li Zhi-ning, Han Wei, Rong Liang-qun, Gong Ai-ping, Lv You, Shan Jun-jun, Wei Xiu-e. Influence of angiogenesis on neural stem cell proliferation in the subventricular zone after focal cerebral ischemia/reperfusion[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(29): 4697-4702.

Figures/Tables 2

2.1 实验动物数量分析实验共使用成年雄性SD大鼠240只，实验过程动物无死亡，全部纳入结果分析。 2.2 局部脑缺血再灌注后各组大鼠脑室下区vWF mRNA表达与正常组及假手术组比较，手术组个时间点的vWF mRNA表达明显增加，差异有显著性意义(P < 0.01)。在缺血1 d时，vWF mRNA表达即增加，7 d时达高峰，14 d时表达逐渐降低。缺血再灌注组各时间点vWF mRNA表达差异有显著性意义(P < 0.01)，见表1。 2.3 局部脑缺血再灌注后各组大鼠脑室下区Nestin的表达手术组在缺血再灌注后Nestin的表达量逐渐增高，至 7 d时达高峰，之后逐渐下降。各组间差异有显著性意义(P < 0.05)。与正常组及假手术组比较，差异有显著性意义(P < 0.05)，见图1，表2。 2.4 侧脑室注射生理盐水或血管内皮生长因子大鼠脑室下区Nestin的表达侧脑室注射血管内皮生长因子+手术组及侧脑室注射生理盐水+手术组在缺血再灌注后Nestin的表达量逐渐增高，至7 d时达高峰，之后逐渐下降。各组间差异有显著性意义(P < 0.05)。与手术组及侧脑室注射生理盐水+手术组比较，侧脑室注射血管内皮生长因子+手术组各时间点Nestin表达量明显增加，差异有显著性意义(P < 0.05)。手术组与侧脑室注射生理盐水+手术组比较，Nestin的表达差异无显著性意义(P > 0.05)，见图2，表3。 2.5 侧脑室注射生理盐水或血管内皮生长因子大鼠脑室下区vWF mRNA的表达侧脑室注射血管内皮生长因子+手术组及侧脑室注射生理盐水+手术组在缺血再灌注后vWF mRNA的表达量逐渐增高，至7 d时达高峰，之后逐渐下降。各组间差异有显著性意义(P < 0.01)。与手术组及侧脑室注射生理盐水+手术组比较，侧脑室注射血管内皮生长因子+手术组各时间点vWF mRNA表达量明显增加，差异有显著性意义(P < 0.01)。手术组与侧脑室注射生理盐水+手术组比较，vWF mRNA表达差异无显著性意义(P > 0.01)，见表4。"

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